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Cytolytic replicati...
Cytolytic replication of coxsackievirus B2 in CAR-deficient rhabdomyosarcoma cells.
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- Polacek, Charlotta (author)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
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- Ekström, Jens-Ola (author)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
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- Lundgren, Anneli (author)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
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- Lindberg, A Michael (author)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
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(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- English.
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In: Virus Research. - : Elsevier BV. - 0168-1702 .- 1872-7492. ; 113:2, s. 107-15
- Related links:
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http://www.sciencedi...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The six coxsackievirus B serotypes (CVB1-6) use the coxsackie- and adenovirus receptor (CAR) for host cell entry. Four of these serotypes, CVB1, 3, 5 and 6, have also shown the capacity to replicate and cause cytolysis in rhabdomyosarcoma (RD) cells, a CAR-deficient cell line. This extended tropism has been associated with an acquired ability to bind decay accelerating factor (DAF). In this study, we have adapted the CVB2 prototype strain Ohio-1 (CVB2/O) to replicate in RD cells. Two types of infection were identified: (I) an enterovirus-typical, lytic infection, and (II) a non-lytic infection. Both CVB2/O-RD variants retained the prototype-ability to cause cytopathic effect in HeLa cells using CAR as receptor. Phenotypic and genotypic changes in the CVB2/O-RD-variants were determined and compared to the prototype cultured in HeLa cells. Inhibition studies using antibodies against CAR and DAF revealed a maintained ability of the CVB2/O-RD-variants to bind CAR, but no binding to DAF was observed. In addition, neither the prototype nor the CVB2/O-RD-variants were able to cause hemagglutination in human red blood cells, an enterovirus feature associated with affinity for DAF. Sequence analysis of the CVB2/O-RD-variants showed acquired mutations in the capsid region, suggesting extended receptor usage towards an alternative, yet unidentified, receptor for CVB2.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Enterovirus
- Receptor
- CAR
- DAF
- Tissue tropism
- Microbiology
- Mikrobiologi
- Biomedicinsk vetenskap
- Biomedical Sciences
Publication and Content Type
- ref (subject category)
- art (subject category)
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