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Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins
- Lee, T W R (author)
- Lawrence, F J (author)
-
- Dauksaite, V (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Blair, G E (author)
- Matthews, D A (author)
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- (creator_code:org_t)
- Microbiology Society, 2004
- 2004
- English.
- In: Journal of General Virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 85:Pt 1, s. 185-196
- Journal article (peer-reviewed)
Abstract
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- We have examined the subcellular localization properties of human adenovirus 2 (HAdV-2) preMu and mature Mu (pX) proteins as fusions with enhanced green fluorescence protein (EGFP). We determined that preMu is exclusively a nucleolar protein with a single nucleolar accumulation signal within the Mu sequence. In addition, we noted that both preMu-EGFP and Mu-EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu-EGFP (but not Mu-EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes. Further investigation suggested this might be due to a failure to express pre-terminal protein (preTP) from the E2 region, despite expression of another E2 protein, DBP. Deletion mutagenesis identified a highly conserved region in the C terminus of preMu responsible for these observations. Thus our data suggest that preMu may play a role in modulating accumulation of proteins from the E2 region.
Keyword
- Adenovirus E2 Proteins/*metabolism
- Adenoviruses; Human/pathogenicity
- Amino Acid Sequence
- Cell Nucleolus/*metabolism
- DNA-Binding Proteins/*metabolism
- Gene Deletion
- Gene Expression Regulation; Viral
- Green Fluorescent Proteins
- Hela Cells
- Humans
- Luminescent Proteins/genetics/metabolism
- Molecular Sequence Data
- Peptides/*metabolism
- Protein Precursors/*metabolism
- Recombinant Fusion Proteins/metabolism
- Research Support; Non-U.S. Gov't
- Viral Core Proteins/*metabolism
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- ref (subject category)
- art (subject category)
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- Lee, T W R
- Lawrence, F J
- Dauksaite, V
- Akusjärvi, Göran
- Blair, G E
- Matthews, D A
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