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A new approach to e...
A new approach to evaluate regression models during validation of bioanalytical assays
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Singtoroj, T (author)
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- Tärning, Joel, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för farmakologi,Institute of Neuroscience and Physiology, Department of Pharmacology
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Annerberg, A (author)
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- Ashton, Michael, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för farmakologi,Institute of Neuroscience and Physiology, Department of Pharmacology
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- Bergqvist, Yngve (author)
- Högskolan Dalarna,Kemiteknik
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White, NJ (author)
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Lindegårdh, N (author)
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Day, NP (author)
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
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In: J Pharm Biomed Anal. - : Elsevier BV. ; 41:1, s. 219-227
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Abstract
Subject headings
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- The quality of bioanalytical data is highly dependent on using an appropriate regression model for calibration curves. Non-weighted linear regression has traditionally been used but is not necessarily the optimal model. Bioanalytical assays generally benefit from using either data transformation and/or weighting since variance normally increases with concentration. A data set with calibrators ranging from 9 to 10000 ng/mL was used to compare a new approach with the traditional approach for selecting an optimal regression model. The new approach used a combination of relative residuals at each calibration level together with precision and accuracy of independent quality control samples over 4 days to select and justify the best regression model. The results showed that log-log transformation without weighting was the simplest model to fit the calibration data and ensure good predictability for this data set.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- bioanalys
- bioanalytical assays; calibration; transformation; weighting; piperaquine; linear regression; Liquid chromatography
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Singtoroj, T
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Tärning, Joel, 1 ...
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Annerberg, A
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Ashton, Michael, ...
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Bergqvist, Yngve
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White, NJ
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show more...
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Lindegårdh, N
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Day, NP
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Pharmaceutical S ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
- J Pharm Biomed A ...
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Journal of Pharm ...
- By the university
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University of Gothenburg
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Högskolan Dalarna