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Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein
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- Hillström, Anna (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences
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- Hagman, Ragnvi (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences
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- Tvedten, Harold (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences
- (creator_code:org_t)
- 2014-05-05
- 2014
- English.
- In: Veterinary Clinical Pathology. - : Wiley. - 0275-6382 .- 1939-165X. ; 43, s. 235-243
- Journal article (peer-reviewed)
Abstract
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- Background Measurement of C-reactive protein (CRP) is used for diagnosing and monitoring systemic inflammatory disease in canine patients. An automated human immunoturbidimetric assay has been validated for measuring canine CRP, but cross-reactivity with canine CRP is unpredictable. Objective The purpose of the study was to validate a new automated canine-specific immunoturbidimetric CRP method (Gentian cCRP). Methods Studies of imprecision, accuracy, prozone effect, interference, limit of quantification, and stability under different storage conditions were performed. The new method was compared with a human CRP assay previously validated for canine CRP determination. Samples from 40 healthy dogs were analyzed to establish a reference interval. Results Total imprecision was < 2.4% for 4 tested serum pools analyzed twice daily over 10days. The method was linear under dilution, and no prozone effect was detected at a concentration of 1200mg/L. Recovery after spiking serum with purified canine CRP at 2 different concentrations was 123% and 116%, respectively. No interference from hemoglobin or triglycerides (10g/L) was detected. CRP was stable for 14days at 4 degrees C and 22 degrees C. In the method comparison study, there was good agreement between the validated human CRP assay and the new canine-specific assay. Healthy dogs had CRP concentrations that were less than the limit of quantification of the Gentian cCRP method (6.8mg/L). Conclusions The new canine-specific immunoturbidimetric CRP assay is a reliable and rapid method for measuring canine CRP, suitable for clinical use due to the option for an automated assay.
Subject headings
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Klinisk vetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Clinical Science (hsv//eng)
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