Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout

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Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout

Sepehri, Sobhan, 1986 (author): Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden

Eriksson, Emil (author): RISE,Acreo

Kalaboukhov, Alexei, 1975 (author): Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden

Zardán Gómez de la Torre, Teresa (author): Uppsala universitet,Nanoteknologi och funktionella material,Nanoteknologi och funktionella material, Nanotechnology and Functional Materials

Kustanovich, Kiryl, 1987 (author): Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden

Jesorka, Aldo, 1967 (author): Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden

Schneiderman, Justin, 1979 (author): Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience,Chalmers University of Technology, Sweden ; University of Gothenburg, Sweden

Blomgren, Jakob (author): RISE,Acreo

Johansson, Christer, 1964 (author): RISE,Acreo

Strömme, Maria, 1970- (author): Uppsala universitet,Nanoteknologi och funktionella material

Winkler, Dag, 1957 (author): Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden

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AIP Publishing, 2018
2018
English.
In: APL Bioengineering. - : AIP Publishing. - 2473-2877. ; 2:1

Related links:: https://research.cha... (primary) (free); show more...; https://aip.scitatio...; https://doi.org/10.1...; https://doi.org/10.1...; https://research.cha...; https://research.cha...; https://gup.ub.gu.se...; https://urn.kb.se/re...; https://urn.kb.se/re...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

A bioassay based on a high-Tc superconducting quantum interference device (SQUID) reading out functionalized magnetic nanoparticles (fMNPs) in a prototype microfluidic platform is presented. The target molecule recognition is based on volume amplification using padlock-probe-ligation followed by rolling circle amplification (RCA). The MNPs are functionalized with single-stranded oligonucleotides, which give a specific binding of the MNPs to the large RCA coil product, resulting in a large change in the amplitude of the imaginary part of the ac magnetic susceptibility. The RCA products from amplification of synthetic Vibrio cholera target DNA were investigated using our SQUID ac susceptibility system in microfluidic channel with an equivalent sample volume of 3 μl. From extrapolation of the linear dependence of the SQUID signal versus concentration of the RCA coils, it is found that the projected limit of detection for our system is about 1.0 e5 RCA coils (0.2e−18 mol), which is equivalent to 66 fM in the 3 μl sample volume. This ultra-high magnetic sensitivity and integration with microfluidic sample handling are critical steps towards magnetic bioassays for rapid detection of DNA and RNA targets at the point of care.

Subject headings

NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Medicinsk bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Medical Biotechnology (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Nanoteknik -- Nanoteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Nano-technology -- Nano-technology (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk apparatteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Equipment Engineering (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Medicinteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Medical Engineering (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Annan materialteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Other Materials Engineering (hsv//eng)

Keyword

POC
high-Tc SQUID
microfluidics
superconductivity
Bioassay
bioassay
high-Tc superconducting sensor
SQUID
functionalized magnetic nanoparticles
MNPs
microfluidics
Teknisk fysik med inriktning mot nanoteknologi och funktionella material

Publication and Content Type

art (subject category)
ref (subject category)

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