A novel combination of TaqMan RT-PCR and a suspension microarray assay for the detection and species identification of pestiviruses

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Leijon, Mikael (författare): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,National Veterinary Institute (SVA)

Jobs, Magnus (författare): Högskolan Dalarna,Uppsala universitet,Klinisk virologi,Medicinsk vetenskap

Belak, Sandor (författare): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,National Veterinary Institute (SVA)

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Elsevier BV, 2010
2010
Engelska.
Ingår i: Veterinary Microbiology. - : Elsevier BV. - 0378-1135 .- 1873-2542. ; 142:1-2, s. 81-86

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Abstract Ämnesord

Stäng

The genus pestivirus contains four recognized species: classical swine fever virus, border disease virus, bovine viral diarrhoea virus types 1 and 2. All are economically important and globally distributed but classical swine fever is the most serious, concerning losses and control measures. It affects both domestic pigs and wild boars. Outbreaks of this disease in domestic pigs call for the most serious measures of disease control, including a stamping out policy in Europe. Since all the members of the pestivirus genus can infect swine, differential diagnosis using traditional methods poses some problems. Antibody tests may lack specificity due to cross-reactions, antigen capture ELISAs may have low sensitivity, and virus isolation may take several days or even longer time to complete. PCR-based tests overcome these problems for the most part, but in general lack the multiplexing capability to detect and differentiate all the pestiviruses simultaneously. The assay platform described here addresses all of these issues by combining the advantages of real-time PCR with the multiplexing capability of microarray technology. The platform includes a TaqMan real-time PCR designed for the universal detection of pestiviruses and a microarray assay that can use the amplicons produced in the real-time PCR to identify the specific pestivirus.

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Patobiologi (hsv//swe)
AGRICULTURAL SCIENCES -- Veterinary Science -- Pathobiology (hsv//eng)
LANTBRUKSVETENSKAPER -- Veterinärmedicin (hsv//swe)
AGRICULTURAL SCIENCES -- Veterinary Science (hsv//eng)
LANTBRUKSVETENSKAPER -- Husdjursvetenskap (hsv//swe)
AGRICULTURAL SCIENCES -- Animal and Dairy Sience (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)

Av författaren/redakt...: LeBlanc, Neil; Leijon, Mikael; Jobs, Magnus; Blomberg, Jonas; Belak, Sandor

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Mikrobiologi ino ...

LANTBRUKSVETENSKAPER: LANTBRUKSVETENSK ...; och Veterinärmedicin; och Patobiologi

Av lärosätet: Uppsala universitet; Sveriges Lantbruksuniversitet; Högskolan Dalarna

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