Microscopic and spectroscopic characterization of rice and corn starch

Govindaraju, Indira (author)

Pallen, Sparsha (author)

Umashankar, Suchitta (author)

Mal, Sib Sankar (author)

Kaniyala Melanthota, Sindhoora (author)

Zhuo, Guan-Yu (author)

Mahato, Krishna Kishore (author)

Mazumder, Nirmal (author)

(creator_code:org_t)

2020-01-08
2020
English.
In: Microscopy research and technique (Print). - : John Wiley & Sons. - 1059-910X .- 1097-0029. ; 83:5, s. 490-498

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Starch granules from rice and corn were isolated, and their molecular mechanism on interaction with alpha-amylase was characterized through biochemical test, microscopic imaging, and spectroscopic measurements. The micro-scale structure of starch granules were observed under an optical microscope and their average size was in the range 1-100 mu m. The surface topological structures of starch with micro-holes due to the effect of alpha- amylase were also visualized under scanning electron microscope. The crystallinity was confirmed by X-ray diffraction patterns as well as second-harmonic generation microscopy. The change in chemical bonds before and after hydrolysis of the starch granules by alpha- amylase was determined by Fourier transform infrared spectroscopy. Combination of microscopy and spectroscopy techniques relates structural and chemical features that explain starch enzymatic hydrolysis which will provide a valid basis for future studies in food science and insights into the energy transformation dynamics.

By the author/editor: Govindaraju, Ind ...; Pallen, Sparsha; Umashankar, Such ...; Mal, Sib Sankar; Kaniyala Melanth ...; Mahato, Dhani Ra ...; show more...; Zhuo, Guan-Yu; Mahato, Krishna ...; Mazumder, Nirmal; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Materials Chemis ...

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