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Testing mixing rule...
Testing mixing rules for structural and dynamical quantities in multi-component crowded protein solutions
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- Gulotta, Alessandro (författare)
- Lund Univ, Div Phys Chem, Nat Vetarvagen 14, S-22100 Lund, Sweden
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- Bucciarelli, Saskia (författare)
- Lund Univ, Div Phys Chem, Nat Vetarvagen 14, S-22100 Lund, Sweden
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- Roosen-Runge, Felix (författare)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Lund Univ, Div Phys Chem, Nat Vetarvagen 14, S-22100 Lund, Sweden
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- Holderer, Olaf (författare)
- Forschungszentrum Julich GmbH, Julich Ctr Neutron Sci JCNS, Heinz Maier Leibnitz Zent MLZ, Garching, Germany
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- Schurtenberger, Peter (författare)
- Lund Univ, Div Phys Chem, Nat Vetarvagen 14, S-22100 Lund, Sweden; Lund Univ, LINXS Inst Adv Neutron & Xray Sci, Lund, Sweden
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- Stradner, Anna (författare)
- Lund Univ, Div Phys Chem, Nat Vetarvagen 14, S-22100 Lund, Sweden; Lund Univ, LINXS Inst Adv Neutron & Xray Sci, Lund, Sweden
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(creator_code:org_t)
- American Institute of Physics (AIP), 2024
- 2024
- Engelska.
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Ingår i: APL BIOENGINEERING. - : American Institute of Physics (AIP). - 2473-2877. ; 8:2
- Relaterad länk:
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https://doi.org/10.1...
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https://mau.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Crowding effects significantly influence the phase behavior and the structural and dynamic properties of the concentrated protein mixtures present in the cytoplasm of cells or in the blood serum. This poses enormous difficulties for our theoretical understanding and our ability to predict the behavior of these systems. While the use of course grained colloid-inspired models allows us to reproduce the key physical solution properties of concentrated monodisperse solutions of individual proteins, we lack corresponding theories for complex polydisperse mixtures. Here, we test the applicability of simple mixing rules in order to predict solution properties of protein mixtures. We use binary mixtures of the well-characterized bovine eye lens proteins alpha and gamma(B) crystallin as model systems. Combining microrheology with static and dynamic scattering techniques and observations of the phase diagram for liquid-liquid phase separation, we show that reasonably accurate descriptions are possible for macroscopic and mesoscopic signatures, while information on the length scale of the individual protein size requires more information on cross-component interaction.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
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