WFRF:(All Ericsson Charlotta)
Sökning: WFRF:(All Ericsson Charlotta) > c-Kit-dependent gro...
- 1 av 9
- Föregående post
- Nästa post
- Till träfflistan
c-Kit-dependent growth of uveal melanoma cells : a potential therapeutic target?
-
- All-Ericsson, Charlotta (författare)
- Karolinska Institutet
-
- Girnita, Leonard (författare)
- Karolinska Institutet
- Müller-Brunotte, Anja (författare)
- visa fler...
- visa färre...
- (creator_code:org_t)
- Association for Research in Vision and Ophthalmology (ARVO), 2004
- 2004
- Engelska.
- Ingår i: Investigative Ophthalmology and Visual Science. - : Association for Research in Vision and Ophthalmology (ARVO). - 0146-0404 .- 1552-5783. ; 45:7, s. 2075-82
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- PURPOSE: This study was conducted to investigate the expression and functional impact of the proto-oncogene c-kit in uveal melanoma. METHODS: Based on immunohistochemical (IHC) study of paraffin-embedded specimens from 134 uveal melanomas and Western blot analysis on eight fresh-frozen samples the expression of c-kit in uveal melanoma was studied. Furthermore, the phosphorylation of c-kit and the impact of the tyrosine kinase inhibitor STI571 was examined in the three uveal melanoma cell lines OCM-1, OCM-3, and 92-1. RESULTS: Eighty-four of 134 paraffin-embedded samples and six of eight fresh-frozen samples expressed c-kit. c-Kit was strongly expressed and tyrosine phosphorylated in cultured uveal melanoma cells compared with cutaneous melanoma cells. Moreover, in contrast to cutaneous melanoma cell lines c-kit maintained a high phosphorylation level in serum-depleted uveal melanoma cells. No activation-related mutations in exon 11 of the KIT gene were found. On the contrary, expression of the stem cell growth factor (c-kit ligand) was detected in all three uveal melanoma cell lines, suggesting the presence of autocrine (paracrine) stimulation pathways. Treatment of uveal melanoma cell lines with STI571, which blocks c-kit autophosphorylation, resulted in cell death. The IC(50) of the inhibitory effects on c-kit phosphorylation and cell proliferation was of equal size and less than 2.5 microM. CONCLUSIONS: The results confirm that c-kit is vastly expressed in uveal melanoma, suggest that the c-kit molecular pathway may be important in uveal melanoma growth, and point to its use as a target for therapy with STI571.
Nyckelord
- Adult
- Aged
- Aged; 80 and over
- Blotting; Western
- Cell Division
- Female
- Humans
- Immunoenzyme Techniques
- Male
- Melanoma/*metabolism/*pathology
- Middle Aged
- Paraffin Embedding
- Phosphorylation/drug effects
- Polymerase Chain Reaction
- Proto-Oncogene Proteins c-kit/genetics/*metabolism
- Pyrimidines/pharmacology
- RNA; Messenger/metabolism
- Skin Neoplasms/metabolism/pathology
- Tumor Cells; Cultured
- Tyrosine/metabolism
- Uveal Neoplasms/*metabolism/*pathology
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
- Investigative Ophthalmology and Visual Science (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- 1 av 9
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- All-Ericsson, Ch ...
- Girnita, Leonard
- Müller-Brunotte, ...
- Brodin, Bertha
- Seregard, Stefan
- Östman, Arne
- visa fler...
- Larsson, Olle
- visa färre...
- Artiklar i publikationen
- Investigative Op ...
- Av lärosätet
- Uppsala universitet
- Karolinska Institutet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
