Impaired Toll-like receptor 2 signaling in monocytes from 5-year-old allergic children

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Amoudruz, PetraStockholms universitet,Wenner-Grens institut (author)

Impaired Toll-like receptor 2 signaling in monocytes from 5-year-old allergic children

Article/chapterEnglish2009

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2009-02-02
Oxford University Press (OUP),2009
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LIBRIS-ID:oai:DiVA.org:su-25188
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-25188URI
https://doi.org/10.1111/j.1365-2249.2008.03792.xDOI
http://kipublications.ki.se/Default.aspx?queryparsed=id:118212845URI

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Language:English
Summary in:English

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Subject category:art swepub-publicationtype

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The relative composition of the two major monocytic subsets CD14+CD16− and CD14+CD16+ is altered in some allergic diseases. These two subsets display different patterns of Toll-like receptor levels, which could have implications for activation of innate immunity leading to reduced immunoglobulin E-specific adaptive immune responses. This study aimed to investigate if allergic status at the age of 5 years is linked to differences in monocytic subset composition and their Toll-like receptor levels, and further, to determine if Toll-like receptor regulation and cytokine production upon microbial stimuli is influenced by the allergic phenotype. Peripheral blood mononuclear cells from 5-year-old allergic and non-allergic children were stimulated in vitro with lipopolysaccharide and peptidoglycan. Cells were analysed with flow cytometry for expression of CD14, Toll-like receptors 2 and 4 and p38-mitogen-activated protein kinase (MAPK). The release of cytokines and chemokines [tumour necrosis factor, interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70] into culture supernatants was measured with cytometric bead array. For unstimulated cells there were no differences in frequency of the monocytic subsets or their Toll-like receptor levels between allergic and non-allergic children. However, monocytes from allergic children had a significantly lower up-regulation of Toll-like receptor 2 upon peptidoglycan stimulation. Further, monocytes from allergic children had a higher spontaneous production of IL-6, but there were no differences between the two groups regarding p38-MAPK activity or cytokine and chemokine production upon stimulation. The allergic subjects in this study have a monocytic population that seems to display a hyporesponsive state as implicated by impaired regulation of Toll-like receptor 2 upon peptidoglycan stimulation.

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Holmlund, UlrikaStockholms universitet,Wenner-Grens institut(Swepub:su)ulho0917 (author)
Saghafian-Hedengren, ShanieStockholms universitet,Wenner-Grens institut(Swepub:su)shsa1905 (author)
Nilsson, CarolineKarolinska Institutet (author)
Sverremark-Ekström, EvaStockholms universitet,Wenner-Grens institut(Swepub:su)esver (author)
Stockholms universitetWenner-Grens institut (creator_code:org_t)

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In:Clinical and Experimental Immunology: Oxford University Press (OUP)155:3, s. 387-3940009-91041365-2249

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By the author/editor: Amoudruz, Petra; Holmlund, Ulrika; Saghafian-Hedeng ...; Nilsson, Carolin ...; Sverremark-Ekstr ...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

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By the university: Stockholm University; Karolinska Institutet

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