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Calcium activates p...
Calcium activates purified human TRPA1 with and without its N-terminal ankyrin repeat domain in the absence of calmodulin
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- Moparthi, Lavanya (författare)
- Linköpings universitet,Linköping University,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten
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- Moparthi, Satish Babu (författare)
- Pasteur Institute,Inst Pasteur, France
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- Wenger, Jérôme (författare)
- Institut Fresnel,Aix-Marseille University,Aix Marseille Univ, France
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- Zygmunt, Peter M. (författare)
- Lund University,Lunds universitet,Neurofarmakologi och smärta,Forskargrupper vid Lunds universitet,Neuropharmacology and pain,Lund University Research Groups,Lund Univ, Sweden
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- Engelska.
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Ingår i: Cell Calcium. - : Elsevier BV. - 0143-4160 .- 1532-1991. ; 90
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://doi.org/10.1...
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https://liu.diva-por... (primary) (Raw object)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Extracellular influx of calcium or release of calcium from intracellular stores have been shown to activate mammalian TRPA1 as well as to sensitize and desensitize TRPA1 electrophilic activation. Calcium binding sites on both intracellular N- and C-termini have been proposed. Here, we demonstrate based on Förster resonance energy transfer (FRET) and bilayer patch-clamp studies, a direct calmodulin-independent action of calcium on the purified human TRPA1 (hTRPA1), causing structural changes and activation without immediate subsequent desensitization of hTRPA1 with and without its N-terminal ankyrin repeat domain (N-ARD). Thus, calcium alone activates hTRPA1 by a direct interaction with binding sites outside the N-ARD.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Calcium
- Calmodulin
- Pain
- TRP channel
- TRPA1
- Calcium; Calmodulin; TRP channel; TRPA1; Pain
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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