Toward Plasmonic Biosensors Functionalized by a Photoinduced Surface Reaction

Gschneidtner, Tina, 1985 (author): Chalmers tekniska högskola,Chalmers University of Technology

Chen, Si, 1985 (author): Chalmers tekniska högskola,Chalmers University of Technology

Christensen, J. B. (author): Köpenhamns universitet,University of Copenhagen

Käll, Mikael, 1963 (author): Chalmers tekniska högskola,Chalmers University of Technology

Moth-Poulsen, Kasper, 1978 (author): Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

2013-07-03
2013
English.
In: Journal of Physical Chemistry C. - : American Chemical Society (ACS). - 1932-7447 .- 1932-7455. ; 117:28, s. 14751-14758

Related links:: http://dx.doi.org/10...; show more...; https://doi.org/10.1...; https://research.cha...; show less...

Abstract Subject headings

We present a method for efficient coupling of amine nucleophilic molecules of choice to a nanostructured gold surface via photoinduced surface chemistry. The method is based on photoactive self-assembled monolayers and can be used to functionalize localized surface plasmon resonance (LSPR) based biosensors with biorecognition motifs while reducing nonspecific binding via introduction of hydrophilic units. The photoactive linker molecule, 5-bromo-7-nitroindoline, couples nucleophilic molecules such as biotin ethylenediamine to a surface when exposed to UV-light. The specific, noncovalent recognition between biotin and streptavidin is used for demonstration of a simple biorecognition assay based on the LSPR sensing principle. By doing so, one can envision that the binding of any streptavidin fusion protein, being attached to specific spots at the gold surface, is monitored by an LSPR peak shift. Since the surface functionalization is based on a photoinduced reaction, this method can be used to functionalize the surface in a local and site-specific way, and biomedical applications such as drug-screening platforms, microarrays, solid support protein synthesis, and even single molecule experiments can be envisioned.

By the author/editor: Gschneidtner, Ti ...; Chen, Si, 1985; Christensen, J. ...; Käll, Mikael, 19 ...; Moth-Poulsen, Ka ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Physical Chemist ...

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