Low agreement between radio binding assays in analyzing glutamic acid decarboxylase (GAD65Ab) autoantibodies in patients classified with type 2 diabetes.

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Fältnamn	Indikatorer	Metadata
000		05175naa a2200769 4500
001		oai:gup.ub.gu.se/107456
003		SwePub
008		240528s2009 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:lup.lub.lu.se:4360cdeb-9111-4193-9511-096aab73e607
009		oai:DiVA.org:umu-35866
024	7	a https://gup.ub.gu.se/publication/1074562 URI
024	7	a https://doi.org/10.1080/089169309029117202 DOI
024	7	a https://lup.lub.lu.se/record/14898162 URI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-358662 URI
040		a (SwePub)gud (SwePub)lud (SwePub)umu
041		a eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Daka, Bledar,d 1976u Umeå universitet,Allmänmedicin4 aut0 (Swepub:gu)xdakbl
245	1 0	a Low agreement between radio binding assays in analyzing glutamic acid decarboxylase (GAD65Ab) autoantibodies in patients classified with type 2 diabetes.
264		c 2009-09-09
264	1	b Informa UK Limited,c 2009
338		a electronic2 rdacarrier
520		a Autoantibodies against glutamic acid decarboxylase (GAD65Ab) are used in the classification of diabetes in adults. We assessed the concordance in GAD65 autoantibody levels within subjects between three different GAD65Ab radio binding assays (RBA). Plasma samples from 112 diabetes patients (median age 50 years) initially classified with type 2 diabetes was randomly selected from a local diabetes registry. Coded samples were analyzed with two RBA employing (35)S-labeled GAD65. The first used the pEx9 plasmid (pEx9 RBA), the second employed the pThGAD65 plasmid (pThGAD65 RBA) to label GAD65 by in vitro transcription translation. We also used a commercial kit employing plasmid pGAD17 labelled with (125)I (pGAD17 RBA). Subsequent analyses followed standard procedures. Two different cut-offs for GAD65Ab positivity were used in all three assays. We calculated the correlation, concordance, and agreement between the assays. The proportion of GAD65Ab positivity differed between assays when low cut-offs were used (pEx9 RBA 25%, pThGAD65 RBA 17.9%, and pGAD17 RBA 12.5%, respectively). When high cut-offs were applied, the concordance between the pEx9 RBA and the pThGAD65 RBA was 97.3 while their concordance to the pGAD17 RBA was lower (88.4 and 87.4, respectively). There was a low agreement between both pEx9 RBA and pGAD17 RBA (0.45, 95% CI 0.20-0.70) and between pThGAD65 RBA and pGAD17 RBA (0.43, 95% CI 0.18-0.68). We found discrepancies in determining the GAD65Ab positivity, which constitutes a problem when GAD65Ab are used clinically. Further methodological GAD65Ab assays studies are warranted.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Allmänmedicin0 (SwePub)302242 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex General Practice0 (SwePub)302242 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Reumatologi och inflammation0 (SwePub)302102 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Rheumatology and Autoimmunity0 (SwePub)302102 hsv//eng
653		a Adult
653		a Autoantibodies
653		a blood
653		a Autoimmunity
653		a Diabetes Mellitus
653		a Type 2
653		a diagnosis
653		a immunology
653		a Glutamate Decarboxylase
653		a genetics
653		a immunology
653		a Humans
653		a Middle Aged
653		a Plasmids
653		a Radioligand Assay
653		a methods
653		a Reagent Kits
653		a Diagnostic
653		a Reproducibility of Results
653		a autoimmunity
653		a Type 1 diabetes
653		a type 2 diabetes
653		a immunological technique
653		a Type 1 diabetes
700	1	a Svensson, M.K,d 1965u Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine4 aut0 (Swepub:gu)xsmara
700	1	a Lernmark, Ke4 aut
700	1	a Mincheva-Nilsson, Lucia,d 1951-u Umeå universitet,Klinisk immunologi4 aut0 (Swepub:umu)lumi0001
700	1	a Hallmans, Göranu Umeå universitet,Näringsforskning4 aut0 (Swepub:umu)goha0001
700	1	a Rolandsson, Olovu Umeå universitet,Allmänmedicin4 aut0 (Swepub:umu)olro0005
700	1	a Lernmark, Åkeu Lund University,Lunds universitet,Celiaki och diabetes,Forskargrupper vid Lunds universitet,Celiac Disease and Diabetes Unit,Lund University Research Groups4 aut0 (Swepub:lu)endo-ale
710	2	a Umeå universitetb Allmänmedicin4 org
773	0	t Autoimmunityd : Informa UK Limitedg 42:6, s. 507-14q 42:6<507-14x 1607-842Xx 0891-6934
856	4	u https://gup.ub.gu.se/publication/107456x primaryx freey FULLTEXT
856	4	u http://dx.doi.org/10.1080/08916930902911720y FULLTEXT
856	4 8	u https://gup.ub.gu.se/publication/107456
856	4 8	u https://doi.org/10.1080/08916930902911720
856	4 8	u https://lup.lub.lu.se/record/1489816
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-35866

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Av författaren/redakt...: Daka, Bledar, 19 ...; Svensson, M.K, 1 ...; Lernmark, Ke; Mincheva-Nilsson ...; Hallmans, Göran; Rolandsson, Olov; visa fler...; Lernmark, Åke; visa färre...

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