SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Dralle Henning)
 

Sökning: WFRF:(Dralle Henning) > (1997-1999) > CD97: A dedifferent...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003387naa a2200349 4500
001oai:DiVA.org:uu-51809
003SwePub
008081017s1997 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-518092 URI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Aust, Gabriela4 aut
2451 0a CD97: A dedifferentiation marker in human thyroid carcinomas
264 1c 1997
338 a print2 rdacarrier
520 a CD97 is a dimeric glycoprotein of Mr 75,000-85,000 and 28,000 belonging to a novel subfamily of seven-span transmembrane region leukocyte cell surface molecules. It is expressed abundantly in cells of hematopoietic origin. This is the first report demonstrating the expression of CD97 outside the hematopoetic system. CD97 was studied in normal human and neoplastic follicular epithelium of the thyroid and anaplastic (n = 3) and papillary (n = 1) thyroid carcinoma cell lines. In normal thyroid tissue (n = 11), no immunoreactivity of CD97 could be found, whereas in differentiated thyroid carcinomas (n = 10), CD97 expression was either lacking or low. Eleven of 12 undifferentiated anaplastic carcinomas revealed high CD97 presentation. CD97 was absent or only weakly present in patients with postoperative T1 tumors but increased greatly with the progression to postoperative T4 tumors. CD97 is clearly present in thyroid carcinoma cell lines but only at a very low level in normal human thyrocytes. Quantitation of CD97 cell surface expression levels revealed that C 643 and SW 1736 cells showed a two to four times higher specific antibody-binding capacity than did 8505 C and HTh 74 cells and a nearly 20 times higher specific antibody-binding capacity than normal thyrocytes. Phorbol 12-myristate 13-acetate treatment progressively caused a decrease of CD97 antigen expression in all cell lines to about 30% of their initial levels after 48 h. Immunohistochemical staining of SW 1736 cells revealed that CD97 is located in most of the cell compartments and suggested a CD97 internalization process after phorbol 12-myristate 13-acetate treatment. Semiquantitative reverse transcription-PCR showed a correlation of CD97 mRNA and cell surface CD97 expression level in the cell lines. SW 1736, HTh 74, and 8505 C cells apparently expressed CD97 with alternative glycosylation compared to peripheral lymphocytes, whereas most of the CD97 antigen presented on thyrocytes and C 643 cells had glycosylation sites resembling those of lymphocytes. The data suggest that CD97 expression may be a sensitive marker of dedifferentiation and of lymph node involvement in human thyroid tumors.
653 a MEDICINE
653 a MEDICIN
7001 a Eichler, Wolfram4 aut
7001 a Laue, Sandy4 aut
7001 a Lehmann, Irina4 aut
7001 a Heldin, Nils-Eriku Uppsala universitet,Institutionen för genetik och patologi4 aut
7001 a Lotz, Oliver4 aut
7001 a Scherbaum, Werner A.4 aut
7001 a Dralle, Henning4 aut
7001 a Hoang-Vu, Cuong4 aut
7102 a Uppsala universitetb Institutionen för genetik och patologi4 org
7730 t Cancer Researchg 57:9, s. 1798-1806q 57:9<1798-1806x 0008-5472x 1538-7445
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-51809

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy