Sökning: WFRF:(Eberhardt U) > (2010-2014) > Nuclear ribosomal i...
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000 | 09310naa a2202269 4500 | |
001 | oai:gup.ub.gu.se/156845 | |
003 | SwePub | |
008 | 240528s2012 | |||||||||||000 ||eng| | |
024 | 7 | a https://gup.ub.gu.se/publication/1568452 URI |
024 | 7 | a https://doi.org/10.1073/pnas.11170181092 DOI |
040 | a (SwePub)gu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
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100 | 1 | a Schoch, CL4 aut |
245 | 1 0 | a Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi |
264 | c 2012-03-27 | |
264 | 1 | b Proceedings of the National Academy of Sciences,c 2012 |
520 | a Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation. The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. The nuclear ribosomal small subunit has poor species-level resolution in fungi. ITS will be formally proposed for adoption as the primary fungal barcode marker to the Consortium for the Barcode of Life, with the possibility that supplementary barcodes may be developed for particular narrowly circumscribed taxonomic groups. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biologisk systematik0 (SwePub)106122 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biological Systematics0 (SwePub)106122 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Bioinformatik och systembiologi0 (SwePub)106102 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Bioinformatics and Systems Biology0 (SwePub)106102 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Zoologi0 (SwePub)106082 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Zoology0 (SwePub)106082 hsv//eng |
653 | a Fungi ; barcoding ; ITS | |
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700 | 1 | a Nilsson, R. Henrik,d 1976u Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences4 aut0 (Swepub:gu)xnihen |
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710 | 2 | a Göteborgs universitetb Institutionen för biologi och miljövetenskap4 org |
773 | 0 | t Proceedings of the National Academy of Sciences of the United States of Americad : Proceedings of the National Academy of Sciencesg 109:16, s. 6241-6246q 109:16<6241-6246x 1091-6490 |
773 | 0 | t Proceedings of the National Academy of Sciencesd : Proceedings of the National Academy of Sciencesg 109:16, s. 6241-6246q 109:16<6241-6246x 0027-8424 |
856 | 4 | u https://www.pnas.org/content/pnas/109/16/6241.full.pdf |
856 | 4 8 | u https://gup.ub.gu.se/publication/156845 |
856 | 4 8 | u https://doi.org/10.1073/pnas.1117018109 |
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