Sökning: WFRF:(Fredrikson S.)
> (1987-1989) >
Use of protein G fo...
Use of protein G for preparation and characterization of rabbit antibodies against rat adipose tissue hormone-sensitive lipase
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- Fredrikson, G (författare)
- Lund University
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- Nilsson, S (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Olsson, H (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine
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- Björck, L (författare)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Molekylär patogenes,Forskargrupper vid Lunds universitet,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Molecular Pathogenesis,Lund University Research Groups
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- Akerström, B (författare)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Antioxidationsmedicin,Forskargrupper vid Lunds universitet,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Antioxidation medicine,Lund University Research Groups
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- Belfrage, P (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine
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(creator_code:org_t)
- Elsevier BV, 1987
- 1987
- Engelska.
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Ingår i: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 97:1, s. 65-70
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The newly described immunoglobulin G-binding streptococcal surface protein, protein G, was used to prepare and characterize rabbit antibodies. The antibodies were directed against rat hormone-sensitive lipase, the rate-limiting enzyme in the hydrolysis of the triacylglycerols stored in adipose tissue. Antiserum was obtained after two injections with 20 micrograms enzyme protein, and the immunoglobulin fraction was obtained using a protein G-based solid-phase radioimmunoassay. The hydrolysis of acylglycerols by the enzyme was inhibited by the antibodies, and the enzyme could be efficiently removed from a solution using the antibodies and heat-killed streptococci expressing surface protein G. By Western blot and detection with 125I-protein G, the antibodies were found to selectively bind to hormone-sensitive lipase and to a smaller extent to two minor contaminants, possibly proteolytic fragments of the lipase. The amount of 125I-labelled protein G bound to the lipase on the blot was quantitatively related to the amount of enzyme protein down to the detection limit 10 ng.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Adipose Tissue/enzymology
- Animals
- Antibodies/analysis
- Antigen-Antibody Complex
- Bacterial Proteins/immunology
- Hormones/pharmacology
- Immunoglobulin G/immunology
- Rabbits/immunology
- Radioimmunoassay/methods
- Rats
- Sterol Esterase/analysis
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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