Selection and characterization of HER2/neu-binding affibody ligands

Steffen, Ann-Charlott (author): Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap

Gunneriusson, Elin (author)

Tolmachev, Vladimir (author): Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi

Carlsson, Jörgen (author): Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi

Adams, Gregg (author)

(creator_code:org_t)

2004-06-08
2004
English.
In: Protein Engineering Design & Selection. - : Oxford University Press (OUP). - 1741-0126 .- 1741-0134. ; 17:5, s. 455-462

Related links:: https://academic.oup...; show more...; https://urn.kb.se/re...; https://doi.org/10.1...; https://urn.kb.se/re...; show less...

Abstract Subject headings

Affibody (affibody) ligands that are specific for the extracellular domain of human epidermal growth factor receptor 2 (HER2/neu) have been selected by phage display technology from a combinatorial protein library based on the 58 amino acid residue staphylococcal protein A-derived Z domain. The predominant variants from the phage selection were produced in Escherichia coli, purified by affinity chromatography, and characterized by biosensor analyses. Two affibody variants were shown to selectively bind to the extracellular domain of HER2/neu (HER2-ECD), but not to control proteins. One of the variants, denoted His6-ZHER2/neu:4, was demonstrated to bind with nanomolar affinity (approximately 50 nM) to the HER2-ECD molecule at a different site than the monoclonal antibody trastuzumab. Furthermore, radiolabeled His6-ZHER2/neu:4 affibody showed specific binding to native HER2/neu, overexpressed on the SKBR-3 tumor cell line. Such affibody ligands might be considered in tumor targeting applications for radionuclide diagnostics and therapy of adenocarcinomas such as breast and ovarian cancers.

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

By the author/editor: Wikman, Maria; Steffen, Ann-Cha ...; Gunneriusson, El ...; Tolmachev, Vladi ...; Adams, Gregory P ...; Carlsson, Jörgen; show more...; Ståhl, Stefan; Adams, Gregg; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

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