WFRF:(Hawkins Philip)
Search: WFRF:(Hawkins Philip) > (2005-2009) > Novel effects of th...
- 1 of 1
- Previous record
- Next record
- To hitlist
Novel effects of the prototype translocating Escherichia coli, strain C25 on intestinal epithelial structure and barrier function
- Zareie, Mehri (author)
- Riff, Jason (author)
- Donato, Kevin (author)
- show more...
- McKay, Derek M (author)
- Perdue, Mary H (author)
- Karmali, Mohamed (author)
- Cohen, Mitchell B (author)
- Hawkins, Jennifer (author)
- Sherman, Philip M (author)
- show less...
- (creator_code:org_t)
- Hindawi Limited, 2005
- 2005
- English.
- In: Cellular Microbiology. - : Hindawi Limited. - 1462-5814 .- 1462-5822. ; 7:12, s. 1782-1797
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Intestinal bacteria play an etiologic role in triggering and perpetuating chronic inflammatory bowel disorders. However, the precise mechanisms whereby the gut microflora influences intestinal cell function remain undefined. Therefore, the effects of the non-pathogenic prototype translocating Escherichia coli, strain C25 on the barrier properties of human T84 and Madine-Darby canine kidney type 1 epithelial cells were examined. T-84 cells were also infected with commensal E. coil, strains F18 and HB101, and enterohaemorrhagic E. coli, serotype O157:H7. Strains F18 and HB101 had no effect on transepithelial electrical resistance (TER) of T84 monolayers. By contrast, epithelial cells infected with strain C25 displayed a time-dependent decrease in TER, preceded by an altered distribution of the cytoskeletal protein alpha-actinin, comparable to infection with E. coli O157:H7. E. coli C25 infection also led to activation of nuclear factor κB (NF-κB), interleukin-8 secretion and alterations in localization of claudin-1, but not zona occludens-1 or claudin-4, in T84 cells. There were adherent C25 bacteria on the intact apical surface of infected T84 cells, while mitochondria appeared swollen and vacuolated. These novel findings demonstrate the ability of a translocating commensal bacterium to adhere to and modulate intestinal epithelial barrier function and to induce morphological changes in a manner distinct from the known enteric pathogen, E. coli O157:H7. © 2005 Blackwell Publishing Ltd.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Cellular Microbiology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Zareie, Mehri
- Riff, Jason
- Donato, Kevin
- McKay, Derek M
- Perdue, Mary H
- Söderholm, Johan ...
- show more...
- Karmali, Mohamed
- Cohen, Mitchell ...
- Hawkins, Jennife ...
- Sherman, Philip ...
- show less...
- Articles in the publication
- Cellular Microbi ...
- By the university
- Linköping University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
