Search: WFRF:(Huddleston John)
> (2015) >
Bovine NK-lysin :
Bovine NK-lysin : Copy number variation and functional diversification
-
- Chen, Junfeng (author)
- Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA.
-
- Huddleston, John (author)
- Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA.;Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA.
-
- Buckley, Reuben M. (author)
- Univ Adelaide, Sch Biol Sci, Adelaide, SA 5005, Australia.
-
show more...
-
- Malig, Maika (author)
- Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA.
-
- Lawhon, Sara D. (author)
- Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA.
-
- Skow, Loren C. (author)
- Texas A&M Univ, Coll Vet Med, Dept Vet Integrat Biosci, College Stn, TX 77843 USA.
-
- Lee, Mi Ok (author)
- Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA.
-
- Eichler, Evan E. (author)
- Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA.;Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA.
-
- Andersson, Leif (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Texas A&M Univ, Coll Vet Med, Dept Vet Integrat Biosci, College Stn, TX 77843 USA.;Swedish Univ Agr Sci, Dept Anim Breeding & Genet, SE-75007 Uppsala, Sweden.,Institutionen för husdjursgenetik (HGEN),Department of Animal Breeding and Genetics
-
- Womack, James E. (author)
- Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA.
-
show less...
-
Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA.;Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA. (creator_code:org_t)
-
- 2015-12-14
- 2015
- English.
-
In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 112:52, s. E7223-E7229
- Related links:
-
https://uu.diva-port... (primary) (Raw object)
-
show more...
-
https://www.pnas.org...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://res.slu.se/i...
-
show less...
Abstract
Subject headings
Close
- NK-lysin is an antimicrobial peptide and effector protein in the host innate immune system. It is coded by a single gene in humans and most other mammalian species. In this study, we provide evidence for the existence of four NK-lysin genes in a repetitive region on cattle chromosome 11. The NK2A, NK2B, and NK2C genes are tandemly arrayed as three copies in similar to 30-35-kb segments, located 41.8 kb upstream of NK1. All four genes are functional, albeit with differential tissue expression. NK1, NK2A, and NK2B exhibited the highest expression in intestine Peyer's patch, whereas NK2C was expressed almost exclusively in lung. The four peptide products were synthesized ex vivo, and their antimicrobial effects against both Gram-positive and Gram-negative bacteria were confirmed with a bacteria-killing assay. Transmission electron microcopy indicated that bovine NK-lysins exhibited their antimicrobial activities by lytic action in the cell membranes. In summary, the single NK-lysin gene in other mammals has expanded to a four-member gene family by tandem duplications in cattle; all four genes are transcribed, and the synthetic peptides corresponding to the core regions are biologically active and likely contribute to innate immunity in ruminants.
Subject headings
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biologisk systematik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biological Systematics (hsv//eng)
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Genetik och förädling inom lantbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Genetics and Breeding in Agricultural Sciences (hsv//eng)
Keyword
- NK-lysin
- antimicrobial peptides
- gene family expansion
- segmental duplication
- copy number polymorphism
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Chen, Junfeng
-
Huddleston, John
-
Buckley, Reuben ...
-
Malig, Maika
-
Lawhon, Sara D.
-
Skow, Loren C.
-
show more...
-
Lee, Mi Ok
-
Eichler, Evan E.
-
Andersson, Leif
-
Womack, James E.
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Genetics
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biological Syste ...
-
- AGRICULTURAL SCIENCES
-
AGRICULTURAL SCI ...
-
and Agricultural Bio ...
-
and Genetics and Bre ...
- Articles in the publication
-
Proceedings of t ...
- By the university
-
Uppsala University
-
Swedish University of Agricultural Sciences