Sökning: WFRF:(Itoh Hiroshi) > (2006-2009) > CD47 promotes neuro...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03459naa a2200541 4500 | |
001 | oai:DiVA.org:umu-12289 | |
003 | SwePub | |
008 | 080111s2006 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-122892 URI |
024 | 7 | a https://doi.org/10.1523/JNEUROSCI.3981-06.20062 DOI |
040 | a (SwePub)umu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Murata, Takaaki4 aut |
245 | 1 0 | a CD47 promotes neuronal development through Src- and FRG/Vav2-mediated activation of Rac and Cdc42. |
264 | 1 | c 2006 |
338 | a print2 rdacarrier | |
520 | a The development of axons and dendrites is controlled by small GTP-binding proteins of the Rho family, but the upstream signaling mechanisms responsible for such regulation remain unclear. We have now investigated the role of the transmembrane protein cluster of differentiation 47 (CD47) in this process with hippocampal neurons. CD47-deficient neurons manifested markedly impaired development of dendrites and axons, whereas overexpression of CD47 promoted such development. Interaction of SH2 domain-containing protein tyrosine phosphatase substrate-1 (SHPS-1) with CD47 also induced the formation of dendritic filopodia and spines. These effects of CD47 were prevented by inhibition of either cell division cycle 42 (Cdc42) or Rac. In CD47-deficient neurons, autophosphorylation of Src was markedly reduced. In addition, overexpression of CD47 promoted the autophosphorylation of Src. Inhibition of Src family kinases indeed prevented CD47-promoted dendritic development. Inhibition of either FGD1-related Cdc42-guanine nucleotide exchange factor (GEF) (FRG) or Vav2, which is a GEF for Cdc42 and Rac and is activated by Src, also prevented the effects of CD47 on dendritic development. These results indicate that CD47 promotes development of dendrites and axons in hippocampal neurons in a manner dependent, at least in part, on activation of Cdc42 and Rac mediated by Src as well as by FRG and Vav2. | |
653 | a Animals | |
653 | a Antigens; CD47/*metabolism | |
653 | a Cells; Cultured | |
653 | a Hippocampus/growth & development/metabolism | |
653 | a Humans | |
653 | a Mice | |
653 | a Mice; Knockout | |
653 | a Neurons/*cytology/*metabolism/physiology | |
653 | a Proteins/*physiology | |
653 | a Proto-Oncogene Proteins c-vav/*physiology | |
653 | a cdc42 GTP-Binding Protein/*metabolism | |
653 | a rac GTP-Binding Proteins/*metabolism | |
653 | a src-Family Kinases/*physiology | |
700 | 1 | a Ohnishi, Hiroshi4 aut |
700 | 1 | a Okazawa, Hideki4 aut |
700 | 1 | a Murata, Yoji4 aut |
700 | 1 | a Kusakari, Shinya4 aut |
700 | 1 | a Hayashi, Yuriko4 aut |
700 | 1 | a Miyashita, Motoaki4 aut |
700 | 1 | a Itoh, Hiroshi4 aut |
700 | 1 | a Oldenborg, Per-Arneu Umeå universitet,Histologi med cellbiologi4 aut0 (Swepub:umu)peol0001 |
700 | 1 | a Furuya, Nobuhiko4 aut |
700 | 1 | a Matozaki, Takashi4 aut |
710 | 2 | a Umeå universitetb Histologi med cellbiologi4 org |
773 | 0 | t Journal of Neuroscienceg 26:48, s. 12397-407q 26:48<12397-407x 1529-2401 |
856 | 4 | u http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=17135401&dopt=Citation |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-12289 |
856 | 4 8 | u https://doi.org/10.1523/JNEUROSCI.3981-06.2006 |
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