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Specific detection of L-glutamate in food using flow-injection analysis and enzymatic recycling of substrate
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- Khampha, Wanida (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Yakovleva, Julia (författare)
- Lund University,Lunds universitet,Muskelbiologi,Forskargrupper vid Lunds universitet,Muscle Biology,Lund University Research Groups
- Isarangkul, D (författare)
- visa fler...
- Wiyakrutta, S (författare)
- Meevootisom, V (författare)
- visa färre...
- (creator_code:org_t)
- Elsevier BV, 2004
- 2004
- Engelska.
- Ingår i: Analytica Chimica Acta. - : Elsevier BV. - 1873-4324 .- 0003-2670. ; 518:1-2, s. 127-135
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- A flow injection analysis (FIA) system for specific determination of L-glutamate in food samples based on a bi-enzymatic amplification system has been developed. The content of L-glutamate in the sample was amplified by cycling between L-glutamate dehydrogenase (GIDH) and a novel enzyme, D-phenylglycine aminotransferase (D-PhgAT). In this system, GIDH converts L-glutamate to 2-oxoglutarate with concomitant reduction of NAD(+) to NADH. D-PhgAT transfers an amino group from D-4-hydroxyphenylglycine to 2-oxoglutarate, thus recycling L-glutamate. Accumulation of NADH in the course of the enzymatic recycling was monitored both by fluorescence and UV absorbance and used for quantification of L-glutamate. The assay was characterized by high long-term stability (at least 70 days) and good reproducibility (within-day and between-day RSDs were 4.3-7.3% and 8.9%). The fluorimetric assay was slightly more sensitive with a L-glutamate detection limit of 0.4 muM and linear range of 2.5-50 muM. The assay was specific for L-glutamate, with recoveries between 95-103% in the presence of 17 different amino acids tested one by one. The method was applied to analysis of real food samples and results were correlated with a commercial Boehringer Mannheim assay kit. (C) 2004 Elsevier B.V. All rights reserved.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
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- Khampha, Wanida
- Yakovleva, Julia
- Isarangkul, D
- Wiyakrutta, S
- Meevootisom, V
- Emnéus, Jenny
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