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AMPK-mediated AS160...
AMPK-mediated AS160 phosphorylation in skeletal muscle is dependent on AMPK catalytic and regulatory subunits.
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- Treebak, Jonas T (author)
- Department of Human Physiology, Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark
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- Glund, Stephan (author)
- Department of Molecular Medicine and Surgery, Section Integrative Physiology, Karolinska Institute, Stockholm, Sweden
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- Deshmukh, Atul (author)
- Karolinska Institutet
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- Klein, Ditte K (author)
- Department of Human Physiology, Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark
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- Long, Yun Chau (author)
- Department of Molecular Medicine and Surgery, Section Integrative Physiology, Karolinska Institute, Stockholm, Sweden
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- Jensen, Thomas E (author)
- Department of Human Physiology, Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark
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- Jörgensen, Sebastian B (author)
- Department of Human Physiology, Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark
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- Viollet, Benoit (author)
- René Descartes University, Institute Cochin, Paris, France
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- Andersson, Leif (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Neumann, Dietbert (author)
- Swiss Federal Institute of Technology, Zurich, Switzerland
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- Wallimann, Theo (author)
- Swiss Federal Institute of Technology, Zurich, Switzerland
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- Richter, Erik A (author)
- Department of Human Physiology, Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark
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- Chibalin, Alexander V (author)
- Karolinska Institutet
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- Zierath, Juleen R (author)
- Karolinska Institutet
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- Wojtaszewski, Jörgen F P (author)
- Department of Human Physiology, Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark
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(creator_code:org_t)
- American Diabetes Association, 2006
- 2006
- English.
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In: Diabetes. - : American Diabetes Association. - 0012-1797 .- 1939-327X. ; 55:7, s. 2051-2058
- Related links:
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http://diabetes.diab...
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https://urn.kb.se/re...
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https://doi.org/10.2...
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http://kipublication...
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Abstract
Subject headings
Close
- AMP-activated protein kinase (AMPK) is a heterotrimeric protein that regulates glucose transport mediated by cellular stress or pharmacological agonists such as 5-aminoimidazole-4-carboxamide 1 beta-D-ribonucleoside (AICAR). AS160, a Rab GTPase-activating protein, provides a mechanism linking AMPK signaling to glucose uptake. We show that AICAR increases AMPK, acetyl-CoA carboxylase, and AS160 phosphorylation by insulin-independent mechanisms in isolated skeletal muscle. Recombinant AMPK heterotrimeric complexes (alpha 1 beta 1 gamma l and alpha 2 beta 2 gamma 1) phosphorylate AS160 in a cell-free assay. In mice deficient in AMPK signaling (alpha 2 AMPK knockout [KO], alpha 2 AMPK kinase dead [KD], and gamma 3 AMPK KO), AICAR effects on AS160 phosphorylation were severely blunted, highlighting that complexes containing alpha 2 and gamma 3 are necessary for AICAR-stimulated AS160 phosphorylation in intact skeletal muscle. Contraction-mediated AS160 phosphorylation was also impaired in alpha 2 AMPK KO and KD but not gamma 3 AMPK KO mice. Our results implicate AS160 as a downstream target of AMPK.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- Adenylate Kinase/deficiency/genetics/*metabolism
- Aminoimidazole Carboxamide/analogs & derivatives/pharmacology
- Animals
- Biological Transport
- Catalysis
- GTPase-Activating Proteins/*metabolism
- Glucose/metabolism
- Insulin/pharmacology
- Kinetics
- Mice
- Mice; Knockout
- Muscle; Skeletal/*enzymology
- Phosphorylation
- Protein Subunits/metabolism
- Ribonucleotides/pharmacology
Publication and Content Type
- ref (subject category)
- art (subject category)
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Diabetes
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To the university's database
- By the author/editor
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Treebak, Jonas T
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Glund, Stephan
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Deshmukh, Atul
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Klein, Ditte K
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Long, Yun Chau
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Jensen, Thomas E
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Jörgensen, Sebas ...
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Viollet, Benoit
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Andersson, Leif
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Neumann, Dietber ...
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Wallimann, Theo
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Richter, Erik A
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Chibalin, Alexan ...
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Zierath, Juleen ...
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Wojtaszewski, Jö ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
- Articles in the publication
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Diabetes
- By the university
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Uppsala University
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Karolinska Institutet