WFRF:(Liu Chong)
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Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
- Weierstall, Uwe (author)
- James, Daniel (author)
- Wang, Chong (author)
- show more...
- White, Thomas A. (author)
- Wang, Dingjie (author)
- Liu, Wei (author)
- Spence, John C. H. (author)
- Doak, R. Bruce (author)
- Nelson, Garrett (author)
- Fromme, Petra (author)
- Fromme, Raimund (author)
- Grotjohann, Ingo (author)
- Kupitz, Christopher (author)
- Zatsepin, Nadia A. (author)
- Liu, Haiguang (author)
- Basu, Shibom (author)
- Wacker, Daniel (author)
- Han, Gye Won (author)
- Katritch, Vsevolod (author)
- Boutet, Sebastien (author)
- Messerschmidt, Marc (author)
- Williams, Garth J. (author)
- Koglin, Jason E. (author)
- Klinker, Markus (author)
- Gati, Cornelius (author)
- Shoeman, Robert L. (author)
- Barty, Anton (author)
- Chapman, Henry N. (author)
- Kirian, Richard A. (author)
- Beyerlein, Kenneth R. (author)
- Stevens, Raymond C. (author)
- Li, Dianfan (author)
- Shah, Syed T. A. (author)
- Howe, Nicole (author)
- Caffrey, Martin (author)
- Cherezov, Vadim (author)
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- (creator_code:org_t)
- 2014-02-14
- 2014
- English.
- In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 5, s. 3309-
- Journal article (peer-reviewed)
Abstract
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- Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mu m-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
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- Weierstall, Uwe
- James, Daniel
- Wang, Chong
- White, Thomas A.
- Wang, Dingjie
- Liu, Wei
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- Spence, John C. ...
- Doak, R. Bruce
- Nelson, Garrett
- Fromme, Petra
- Fromme, Raimund
- Grotjohann, Ingo
- Kupitz, Christop ...
- Zatsepin, Nadia ...
- Liu, Haiguang
- Basu, Shibom
- Wacker, Daniel
- Han, Gye Won
- Katritch, Vsevol ...
- Boutet, Sebastie ...
- Messerschmidt, M ...
- Williams, Garth ...
- Koglin, Jason E.
- Seibert, Marvin ...
- Klinker, Markus
- Gati, Cornelius
- Shoeman, Robert ...
- Barty, Anton
- Chapman, Henry N ...
- Kirian, Richard ...
- Beyerlein, Kenne ...
- Stevens, Raymond ...
- Li, Dianfan
- Shah, Syed T. A.
- Howe, Nicole
- Caffrey, Martin
- Cherezov, Vadim
- show less...
- Articles in the publication
- Nature Communica ...
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- Uppsala University
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