WFRF:(Mazari Aslam M. A.)
Sökning: WFRF:(Mazari Aslam M. A.) > (2017) > Expression of a Dro...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03480naa a2200493 4500 | |
| 001 | oai:DiVA.org:su-142613 | |
| 003 | SwePub | |
| 008 | 170522s2017 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1426132 URI |
| 024 | 7 | a https://doi.org/10.1111/nph.143262 DOI |
| 040 | a (SwePub)su | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Tzafestas, Kyriakos4 aut |
| 245 | 1 0 | a Expression of a Drosophila glutathione transferase in Arabidopsis confers the ability to detoxify the environmental pollutant, and explosive, 2,4,6-trinitrotoluene |
| 264 | c 2016-12-06 | |
| 264 | 1 | b Wiley,c 2017 |
| 338 | a print2 rdacarrier | |
| 520 | a The explosive 2,4,6-trinitrotoluene (TNT) is a significant, global environmental pollutant that is both toxic and recalcitrant to degradation. Given the sheer scale and inaccessible nature of contaminated areas, phytoremediation may be a viable clean-up approach. Here, we have characterized a Drosophila melanogaster glutathione transferase (DmGSTE6) which has activity towards TNT. Recombinantly expressed, purified DmGSTE6 produces predominantly 2-glutathionyl-4, 6-dinitrotoluene, and has a 2.5-fold higher Maximal Velocity (Vmax), and five-fold lower Michaelis Constant (Km) than previously characterized TNT-active Arabidopsis thaliana (Arabidopsis) GSTs. Expression of DmGSTE6 in Arabidopsis conferred enhanced resistance to TNT, and increased the ability to remove TNT from contaminated soil relative to wild-type plants. Arabidopsis lines overexpressing TNT-active GSTs AtGST-U24 and AtGST-U25 were compromised in biomass production when grown in the absence of TNT. This yield drag was not observed in the DmGSTE6-expressing Arabidopsis lines. We hypothesize that increased levels of endogenous TNT-active GSTs catalyse excessive glutathionylation of endogenous substrates, depleting glutathione pools, an activity that DmGST may lack. In conclusion, DmGSTE6 has activity towards TNT, producing a compound with potential for further biodegradation. Selecting or manipulating plants to confer DmGSTE6-like activity could contribute towards development of phytoremediation strategies to clean up TNT from polluted military sites. | |
| 650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
| 653 | a 2 | |
| 653 | a 4 | |
| 653 | a 6-trinitrotoluene | |
| 653 | a Arabidopsis | |
| 653 | a conjugation | |
| 653 | a detoxification | |
| 653 | a Drosophila melanogaster | |
| 653 | a environmental pollutant | |
| 653 | a glutathione transferase | |
| 653 | a phytoremediation | |
| 700 | 1 | a Razalan, Maria M.4 aut |
| 700 | 1 | a Gyulev, Ivan4 aut |
| 700 | 1 | a Mazari, Aslam M. A.u Stockholms universitet,Institutionen för neurokemi4 aut |
| 700 | 1 | a Mannervik, Bengtu Stockholms universitet,Institutionen för neurokemi4 aut0 (Swepub:su)bmann |
| 700 | 1 | a Rylott, Elizabeth L.4 aut |
| 700 | 1 | a Bruce, Neil C.4 aut |
| 710 | 2 | a Stockholms universitetb Institutionen för neurokemi4 org |
| 773 | 0 | t New Phytologistd : Wileyg 214:1, s. 294-303q 214:1<294-303x 0028-646Xx 1469-8137 |
| 856 | 4 | u https://eprints.whiterose.ac.uk/109289/1/Tzafestas_et_al_New_Phytologist.pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-142613 |
| 856 | 4 8 | u https://doi.org/10.1111/nph.14326 |
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