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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003388naa a2200505 4500
001oai:DiVA.org:kth-27982
003SwePub
008110103s2010 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-279822 URI
0247 a https://doi.org/10.1016/j.bpj.2010.10.0322 DOI
040 a (SwePub)kth
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Beckham, Gregg T.4 aut
2451 0a The O-Glycosylated Linker from the Trichoderma reesei Family 7 Cellulase Is a Flexible, Disordered Protein
264 1b Elsevier BV,c 2010
338 a print2 rdacarrier
500 a QC 20110103
520 a Fungi and bacteria secrete glycoprotein cocktails to deconstruct cellulose Cellulose degrading enzymes (cellulases) are often modular with catalytic domains for cellulose hydrolysis and carbohydrate binding modules connected by linkers rich in serine and threonine with O-glycosylation Few studies have probed the role that the linker and O-glycans play in catalysis Since different expression and growth conditions produce different glycosylation patterns that affect enzyme activity the structure function relationships that glycosylation imparts to linkers are relevant for understanding cellulase mechanisms Here the linker of the Trichoderma reesei Family 7 cellobiohydrolase (Cel7A) is examined by simulation Our results suggest that the Cel7A linker is an intrinsically disordered protein with and without glycosylation Contrary to the predominant view the O-glycosylation does not change the stiffness of the linker as measured by the relative fluctuations in the end to end distance rather it provides a 16 A extension thus expanding the operating range of Cel7A We explain observations from previous biochemical experiments in the light of results obtained here and compare the Cel7A linker with linkers from other cellulases with sequence based tools to predict disorder This preliminary screen indicates that linkers from Family 7 enzymes from other genera and other cellulases within T reesei may not be as disordered warranting further study
650 7a TEKNIK OCH TEKNOLOGIERx Kemiteknik0 (SwePub)2042 hsv//swe
650 7a ENGINEERING AND TECHNOLOGYx Chemical Engineering0 (SwePub)2042 hsv//eng
653 a Chemical engineering
653 a Kemiteknik
7001 a Bomble, Yannick J.4 aut
7001 a Matthews, James F.4 aut
7001 a Taylor, Courtney B.4 aut
7001 a Resch, Michael G.4 aut
7001 a Yarbrough, John M.4 aut
7001 a Decker, Steve R.4 aut
7001 a Bu, Lintao4 aut
7001 a Zhao, Xiongce4 aut
7001 a McCabe, Clare4 aut
7001 a Wohlert, Jakob4 aut
7001 a Bergenstråhle, Malinu KTH,Wallenberg Wood Science Center4 aut0 (Swepub:kth)u1ji7e96
7001 a Brady, John W.4 aut
7001 a Adney, William S.4 aut
7001 a Himmel, Michael E.4 aut
7001 a Crowley, Michael F.4 aut
7102 a KTHb Wallenberg Wood Science Center4 org
7730 t Biophysical Journald : Elsevier BVg 99:11, s. 3773-3781q 99:11<3773-3781x 0006-3495x 1542-0086
8564 u http://www.cell.com/article/S0006349510013202/pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-27982
8564 8u https://doi.org/10.1016/j.bpj.2010.10.032

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