Search: WFRF:(McClorey Graham) >
Peptide nanoparticl...
Peptide nanoparticle delivery of charge-neutral splice-switching morpholino oligonucleotides.
-
- Järver, Peter (author)
- Medical Research Council , Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom
-
- Zaghloul, Eman M (author)
- Karolinska Institutet
-
- Arzumanov, Andrey A (author)
- Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom
-
show more...
-
- Saleh, Amer F (author)
- Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom
-
- McClorey, Graham (author)
- Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom
-
- Hammond, Suzan M (author)
- Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom
-
- Hällbrink, Mattias (author)
- Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital, Huddinge, Sweden
-
- Langel, Ülo (author)
- Stockholms universitet,Institutionen för neurokemi
-
- Smith, C I Edvard (author)
- Karolinska Institutet
-
- Wood, Matthew J A (author)
- Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom
-
- Gait, Michael J (author)
- Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom
-
- El Andaloussi, Samir (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- Mary Ann Liebert Inc, 2015
- 2015
- English.
-
In: Nucleic Acid Therapeutics. - : Mary Ann Liebert Inc. - 2159-3337 .- 2159-3345. ; 25:2, s. 65-77
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://www.liebertp...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Oligonucleotide analogs have provided novel therapeutics targeting various disorders. However, their poor cellular uptake remains a major obstacle for their clinical development. Negatively charged oligonucleotides, such as 2'-O-Methyl RNA and locked nucleic acids have in recent years been delivered successfully into cells through complex formation with cationic polymers, peptides, liposomes, or similar nanoparticle delivery systems. However, due to the lack of electrostatic interactions, this promising delivery method has been unsuccessful to date using charge-neutral oligonucleotide analogs. We show here that lipid-functionalized cell-penetrating peptides can be efficiently exploited for cellular transfection of the charge-neutral oligonucleotide analog phosphorodiamidate morpholino. The lipopeptides form complexes with splice-switching phosphorodiamidate morpholino oligonucleotide and can be delivered into clinically relevant cell lines that are otherwise difficult to transfect while retaining biological activity. To our knowledge, this is the first study to show delivery through complex formation of biologically active charge-neutral oligonucleotides by cationic peptides.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Järver, Peter
-
Zaghloul, Eman M
-
Arzumanov, Andre ...
-
Saleh, Amer F
-
McClorey, Graham
-
Hammond, Suzan M
-
show more...
-
Hällbrink, Matti ...
-
Langel, Ülo
-
Smith, C I Edvar ...
-
Wood, Matthew J ...
-
Gait, Michael J
-
El Andaloussi, S ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
- Articles in the publication
-
Nucleic Acid The ...
- By the university
-
Stockholm University
-
Karolinska Institutet