Search: WFRF:(Nielsen Elisabet I. 1973 ) >
Predicting mutant s...
Predicting mutant selection in competition experiments with ciprofloxacin-exposed Escherichia coli
-
- Khan, David (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
- Lagerbäck, Pernilla (author)
- Uppsala universitet,Infektionssjukdomar
-
- Malmberg, Christer (author)
- Uppsala universitet,Infektionssjukdomar
-
show more...
-
- Kristoffersson, Anders, 1985- (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
- Gullberg, Erik, 1980- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Cao, Sha (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Cars, Otto (author)
- Uppsala universitet,Infektionssjukdomar
-
- Andersson, Dan I. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Hughes, Diarmaid, 1956- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Nielsen, Elisabet I., 1973- (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
- Friberg, Lena E (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2018
- 2018
- English.
-
In: International Journal of Antimicrobial Agents. - : Elsevier BV. - 0924-8579 .- 1872-7913. ; 51:3, s. 399-406
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Predicting competition between antibiotic-susceptible wild-type (WT) and less susceptible mutant (MT) bacteria is valuable for understanding how drug concentrations influence the emergence of resistance. Pharmacokinetic/pharmacodynamic (PK/PD) models predicting the rate and extent of takeover of resistant bacteria during different antibiotic pressures can thus be a valuable tool in improving treatment regimens. The aim of this study was to evaluate a previously developed mechanism-based PK/PD model for its ability to predict in vitro mixed-population experiments with competition between Escherichia coli (E. coli) WT and three well-defined E. coli resistant MTs when exposed to ciprofloxacin. Model predictions for each bacterial strain and ciprofloxacin concentration were made for in vitro static and dynamic time–kill experiments measuring CFU (colony forming units)/mL up to 24 h with concentrations close to or below the minimum inhibitory concentration (MIC), as well as for serial passage experiments with concentrations well below the MIC measuring ratios between the two strains with flow cytometry. The model was found to reasonably well predict the initial bacterial growth and killing of most static and dynamic time–kill competition experiments without need for parameter re-estimation. With parameter re-estimation of growth rates, an adequate fit was also obtained for the 6-day serial passage competition experiments. No bacterial interaction in growth was observed. This study demonstrates the predictive capacity of a PK/PD model and further supports the application of PK/PD modelling for prediction of bacterial kill in different settings, including resistance selection.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- Ciprofloxacin
- Escherichia coli
- PK/PD modelling
- PK/PD predictions
- Pharmacokinetics/Pharmacodynamics
- Time–kill experiments
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Khan, David
-
Lagerbäck, Perni ...
-
Malmberg, Christ ...
-
Kristoffersson, ...
-
Gullberg, Erik, ...
-
Cao, Sha
-
show more...
-
Cars, Otto
-
Andersson, Dan I ...
-
Hughes, Diarmaid ...
-
Nielsen, Elisabe ...
-
Friberg, Lena E
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Pharmaceutical S ...
- Articles in the publication
-
International Jo ...
- By the university
-
Uppsala University