Molecular Insights into Covalently Stained Carious Dentine Using Solid-State NMR and ToF-SIMS

Almhöjd, Ulrica S. (author): RISE,Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology,Sahlgrenska Academy, Sweden,Kemi och material

Lingström, Peter, 1960 (author): Gothenburg University,Göteborgs universitet,Institutionen för odontologi, sektion 3,Institute of Odontology, Section 3,Sahlgrenska Academy, Sweden

Nilsson, Åke, 1940 (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Sahlgrenska Academy, Sweden

Norén, Jörgen G, 1947 (author): Gothenburg University,Göteborgs universitet,Institutionen för odontologi, sektion 3,Institute of Odontology, Section 3,Sahlgrenska Academy, Sweden

Siljestrom, S. (author)

(creator_code:org_t)

2017-05-13
2017
English.
In: Caries Research. - : S. Karger AG. - 0008-6568 .- 1421-976X. ; 51:3, s. 255-263

Related links:: https://gup.ub.gu.se...; show more...; https://doi.org/10.1...; https://urn.kb.se/re...; show less...

Abstract Subject headings

Dyes currently used to stain carious dentine have a limited capacity to discriminate normal dentine from carious dentine, which may result in overexcavation. Consequently, finding a selective dye is still a challenge. However, there is evidence that hydrazine-based dyes, via covalent bonds to functional groups, bind specifically to carious dentine. The aim of this study was to investigate the possible formation of covalent bonds between carious dentine and N-15(2)-hydrazine and the hydrazine-based dye, N-15(2)-labelled Lucifer Yellow, respectively. Powdered dentine from extracted carious and normal teeth was exposed to the dyes, and the staining reactions were analysed using time-of-flight secondary ion mass spectrometry (ToF-SIMS), solid-state C-13-labelled nuclear magnetic resonance (NMR) and N-15-NMR spectroscopy. The results showed that N-15(2)-hydrazine and N-15(2)-labelled Lucifer Yellow both bind to carious dentine but not to normal dentine. It can thus be concluded that hydrazine-based dyes can be used to stain carious dentine and leave normal dentine unstained. (C) 2017 S. Karger AG, Basel

By the author/editor: Almhöjd, Ulrica ...; Lingström, Peter ...; Nilsson, Åke, 19 ...; Norén, Jörgen G, ...; Siljestrom, S.; Ostlund, A.; show more...; Bernin, D.; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Dentistry

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