Sökning: WFRF:(Padhan Narendra) > (2013) > Tetraspanin CD63 Pr...
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000 | 03444naa a2200373 4500 | |
001 | oai:DiVA.org:uu-205005 | |
003 | SwePub | |
008 | 130813s2013 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2050052 URI |
024 | 7 | a https://doi.org/10.1074/jbc.M113.4681992 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Tugues, Soniau Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut |
245 | 1 0 | a Tetraspanin CD63 Promotes Vascular Endothelial Growth Factor Receptor 2-beta 1 Integrin Complex Formation, Thereby Regulating Activation and Downstream Signaling in Endothelial Cells in Vitro and in Vivo |
264 | 1 | c 2013 |
338 | a print2 rdacarrier | |
520 | a CD63 is a member of the transmembrane-4 glycoprotein superfamily (tetraspanins) implicated in the regulation of membrane protein trafficking, leukocyte recruitment, and adhesion processes. We have investigated the involvement of CD63 in endothelial cell (EC) signaling downstream of beta 1 integrin and VEGF. We report that silencing of CD63 in primary ECs arrested capillary sprouting and tube formation in vitro because of impaired adhesion and migration of ECs. Mechanistically, CD63 associated with both beta 1 integrin and the main VEGF receptor on ECs, VEGFR2. Our data suggest that CD63 serves to bridge between beta 1 integrin and VEGFR2 because CD63 silencing disrupted VEGFR2-beta 1 integrin complex formation identified using proximity ligation assays. Signaling downstream of beta 1 integrin and VEGFR2 was attenuated in CD63-silenced cells, although their cell surface expression levels remained unaffected. CD63 was furthermore required for efficient internalization of VEGFR2 in response to VEGF. Importantly, systemic delivery of VEGF failed to potently induce VEGFR2 phosphorylation and downstream signaling in CD63-deficient mouse lungs. Taken together, our findings demonstrate a previously unrecognized role for CD63 in coordinated integrin and receptor tyrosine kinase signaling in vitro and in vivo. | |
700 | 1 | a Honjo, Satoshiu Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut |
700 | 1 | a König, Christianu Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut |
700 | 1 | a Padhan, Narendrau Uppsala universitet,Cancer och vaskulärbiologi4 aut0 (Swepub:uu)narpa396 |
700 | 1 | a Kroon, Jeffreyu Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut |
700 | 1 | a Gualandi, Laurau Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut |
700 | 1 | a Li, Xiujuanu Uppsala universitet,Cancer och vaskulärbiologi4 aut0 (Swepub:uu)xilix168 |
700 | 1 | a Barkefors, Irmeliu Uppsala universitet,Beräknings- och systembiologi4 aut0 (Swepub:uu)irmba953 |
700 | 1 | a Thijssen, Victor L.4 aut |
700 | 1 | a Griffioen, Arjan W.4 aut |
700 | 1 | a Claesson-Welsh, Lenau Uppsala universitet,Cancer och vaskulärbiologi4 aut0 (Swepub:uu)lcl04207 |
710 | 2 | a Uppsala universitetb Institutionen för immunologi, genetik och patologi4 org |
773 | 0 | t Journal of Biological Chemistryg 288:26, s. 19060-19071q 288:26<19060-19071x 0021-9258x 1083-351X |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-205005 |
856 | 4 8 | u https://doi.org/10.1074/jbc.M113.468199 |
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