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The circular RNome ...
The circular RNome of primary breast cancer
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- Smid, Marcel (author)
- Erasmus University Medical Center
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- Wilting, Saskia M. (author)
- Erasmus University Medical Center
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- Uhr, Katharina (author)
- Erasmus University Medical Center
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- Rodríguez-González, F. Germán (author)
- Erasmus University Medical Center
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- De Weerd, Vanja (author)
- Erasmus University Medical Center
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- Prager-Van Der Smissen, Wendy J.C. (author)
- Erasmus University Medical Center
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- Van Der Vlugt-Daane, Michelle (author)
- Erasmus University Medical Center
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- Van Galen, Anne (author)
- Erasmus University Medical Center
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- Nik-Zainal, Serena (author)
- Cambridge University Hospitals NHS Foundation Trust,Wellcome Trust Sanger Institute
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- Butler, Adam (author)
- Wellcome Trust Sanger Institute
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- Martin, Sancha (author)
- Wellcome Trust Sanger Institute
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- Davies, Helen R. (author)
- Wellcome Trust Sanger Institute
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- Staaf, Johan (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Forskningsgrupp Lungcancer,Forskargrupper vid Lunds universitet,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Research Group Lung Cancer,Lund University Research Groups
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- Van De Vijver, Marc J. (author)
- Academic Medical Center of University of Amsterdam (AMC)
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- Richardson, Andrea L. (author)
- Brigham and Women's Hospital / Harvard Medical School,Dana-Farber Cancer Institute
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- MacGrogan, Gaëten (author)
- Institut Bergoníe
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- Salgado, Roberto (author)
- University of Antwerp,Institut Jules Bordet
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- Van Den Eynden, Gert G.G.M. (author)
- University of Antwerp,Institut Jules Bordet
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- Purdie, Colin A. (author)
- Ninewells Hospital and Medical School
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- Thompson, Alastair M. (author)
- Ninewells Hospital and Medical School
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- Caldas, Carlos (author)
- University of Cambridge
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- Span, Paul N. (author)
- Radboud University Nijmegen
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- Sweep, Fred C.G.J. (author)
- Radboud University Nijmegen
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- Simpson, Peter T. (author)
- University of Queensland
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- Lakhani, Sunil R. (author)
- Royal Brisbane and Women's Hospital,University of Queensland
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- Van Laere, Steven (author)
- University of Antwerp
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- Desmedt, Christine (author)
- Institut Jules Bordet
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- Paradiso, Angelo (author)
- Istituto Tumori 'Giovanni Paolo II'
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- Eyfjord, Jorunn (author)
- University of Iceland
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- Broeks, Annegien (author)
- Netherlands Cancer Institute
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- Vincent-Salomon, Anne (author)
- Curie Institute, Paris
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- Futreal, Andrew P. (author)
- University of Texas
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- Knappskog, Stian (author)
- Haukeland University Hospital,University of Bergen
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- King, Tari (author)
- Memorial Sloan-Kettering Cancer Center
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- Viari, Alain (author)
- National Institute for Research in Computer Science and Control (INIRA),Centre Léon Bérard
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- Børresen-Dale, Anne Lise (author)
- Oslo university hospital,University of Oslo
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- Stunnenberg, Hendrik G. (author)
- Radboud University Nijmegen
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- Stratton, Mike (author)
- Wellcome Trust Sanger Institute
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- Foekens, John A. (author)
- Erasmus University Medical Center
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- Sieuwerts, Anieta M. (author)
- Erasmus University Medical Center
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- Martens, John W.M. (author)
- Erasmus University Medical Center
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(creator_code:org_t)
- 2019-01-28
- 2019
- English 11 s.
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In: Genome Research. - : Cold Spring Harbor Laboratory. - 1088-9051 .- 1549-5469. ; 29:3, s. 356-366
- Related links:
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http://dx.doi.org/10...
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http://genome.cshlp....
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Circular RNAs (circRNAs) are a class of RNAs that is under increasing scrutiny, although their functional roles are debated. We analyzed RNA-seq data of 348 primary breast cancers and developed a method to identify circRNAs that does not rely on unmapped reads or known splice junctions. We identified 95,843 circRNAs, of which 20,441 were found recurrently. Of the circRNAs that match exon boundaries of the same gene, 668 showed a poor or even negative (R <0.2) correlation with the expression level of the linear gene. In silico analysis showed only a minority (8.5%) of circRNAs could be explained by known splicing events. Both these observations suggest that specific regulatory processes for circRNAs exist. We confirmed the presence of circRNAs of CNOT2, CREBBP, and RERE in an independent pool of primary breast cancers. We identified circRNA profiles associated with subgroups of breast cancers and with biological and clinical features, such as amount of tumor lymphocytic infiltrate and proliferation index. siRNA-mediated knockdown of circCNOT2 was shown to significantly reduce viability of the breast cancer cell lines MCF-7 and BT-474, further underlining the biological relevance of circRNAs. Furthermore, we found that circular, and not linear, CNOT2 levels are predictive for progression-free survival time to aromatase inhibitor (AI) therapy in advanced breast cancer patients, and found that circCNOT2 is detectable in cell-free RNA from plasma. We showed that circRNAs are abundantly present, show characteristics of being specifically regulated, are associated with clinical and biological properties, and thus are relevant in breast cancer.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Smid, Marcel
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Wilting, Saskia ...
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Uhr, Katharina
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Rodríguez-Gonzál ...
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De Weerd, Vanja
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Prager-Van Der S ...
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show more...
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Van Der Vlugt-Da ...
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Van Galen, Anne
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Nik-Zainal, Sere ...
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Butler, Adam
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Martin, Sancha
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Davies, Helen R.
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Staaf, Johan
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Van De Vijver, M ...
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Richardson, Andr ...
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MacGrogan, Gaëte ...
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Salgado, Roberto
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Van Den Eynden, ...
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Purdie, Colin A.
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Thompson, Alasta ...
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Caldas, Carlos
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Span, Paul N.
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Sweep, Fred C.G. ...
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Simpson, Peter T ...
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Lakhani, Sunil R ...
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Van Laere, Steve ...
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Desmedt, Christi ...
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Paradiso, Angelo
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Eyfjord, Jorunn
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Broeks, Annegien
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Vincent-Salomon, ...
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Futreal, Andrew ...
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Knappskog, Stian
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King, Tari
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Viari, Alain
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Børresen-Dale, A ...
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Stunnenberg, Hen ...
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Stratton, Mike
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Foekens, John A.
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Sieuwerts, Aniet ...
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Martens, John W. ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Genome Research
- By the university
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Lund University