WFRF:(Purg Miha)
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CADEE : Computer-Aided Directed Evolution of Enzymes
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- Amrein, Beat Anton (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Struktur- och molekylärbiologi
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- Steffen-Munsberg, Fabian (author)
- Uppsala universitet,Struktur- och molekylärbiologi,Science for Life Laboratory, SciLifeLab
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- Szeler, Ireneusz (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Struktur- och molekylärbiologi
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- (creator_code:org_t)
- 2017
- 2017
- English.
- In: IUCrJ. - 2052-2525. ; 4:1, s. 50-64
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Data- och informationsvetenskap -- Bioinformatik (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences -- Bioinformatics (hsv//eng)
- NATURVETENSKAP -- Kemi -- Teoretisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Theoretical Chemistry (hsv//eng)
Keyword
- computational directed evolution
- computational enzyme design
- distributed computing
- empirical valence bond
- triosephosphate isomerase
Publication and Content Type
- ref (subject category)
- art (subject category)
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