The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.
Steffen-Munsberg, FabianUppsala universitet,Struktur- och molekylärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)fabst747
(författare)
Szeler, IreneuszUppsala universitet,Science for Life Laboratory, SciLifeLab,Struktur- och molekylärbiologi
(författare)
Purg, MihaUppsala universitet,Struktur- och molekylärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)mihpu392
(författare)
Kulkarni, YashrajUppsala universitet,Struktur- och molekylärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)yasku118
(författare)
Kamerlin, Shina Caroline LynnUppsala universitet,Struktur- och molekylärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)lynka392
(författare)
Uppsala universitetScience for Life Laboratory, SciLifeLab
(creator_code:org_t)