WFRF:(Rosing J)
Search: WFRF:(Rosing J) > (2015-2019) > Quantification of m...
- 1 of 5
- Previous record
- Next record
- To hitlist
Quantification of miltefosine in peripheral blood mononuclear cells by high-performance liquid chromatography-tandem mass spectrometry.
- Kip, A E (author)
- Rosing, H (author)
- Hillebrand, M J X (author)
- show more...
- Castro, M M (author)
- Gomez, M A (author)
- Schellens, J H M (author)
- Beijnen, J H (author)
- Dorlo, T P C (author)
- show less...
- Elsevier BV, 2015
- 2015
- English.
- In: Journal of chromatography. B. - : Elsevier BV. - 1570-0232 .- 1873-376X. ; 998-999, s. 57-62
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Phagocytes, the physiological compartment in which Leishmania parasites reside, are the main site of action of the drug miltefosine, but the intracellular pharmacokinetics of miltefosine remain unexplored. We developed a bioanalytical method to quantify miltefosine in human peripheral blood mononuclear cells (PBMCs), expanding from an existing high performance liquid chromatography-tandem mass spectrometry method for the quantification of miltefosine in plasma. The method introduced deuterated miltefosine as an internal standard. Miltefosine was extracted from PBMC pellets by addition of 62.5% methanol. Supernatant was collected, evaporated and reconstituted in plasma. Chromatographic separation was performed on a reversed phase C18 column and detection with a triple-quadrupole mass spectrometer. Miltefosine was quantified using plasma calibration standards ranging from 4 to 1000ng/mL. This method was validated with respect to its PBMC matrix effect, selectivity, recovery and stability. No matrix effect could be observed from the PBMC content (ranging from 0.17 to 26.3×10(6)PBMCs) reconstituted in plasma, as quality control samples were within 3.0% of the nominal concentration (precision less than 7.7%). At the lower limit of quantitation of 4 ng/mL plasma, corresponding to 0.12ng/10(6) PBMCs in a typical clinical sample, measured concentrations were within 8.6% of the nominal value. Recovery showed to be reproducible as adding additional pre-treatment steps did not increase the recovery with more than 9%. This method was successfully applied to measure intracellular miltefosine concentrations in PBMC samples from six cutaneous leishmaniasis patients up to one month post-treatment.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Journal of chromatography. B (Search for host publication in LIBRIS)
To the university's database
- 1 of 5
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Kip, A E
- Rosing, H
- Hillebrand, M J ...
- Castro, M M
- Gomez, M A
- Schellens, J H M
- show more...
- Beijnen, J H
- Dorlo, T P C
- show less...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- and Analytical Chemi ...
- Articles in the publication
- Journal of chrom ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
