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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03546naa a2200373 4500 | |
| 001 | oai:DiVA.org:uu-342430 | |
| 003 | SwePub | |
| 008 | 180220s2018 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:137964734 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3424302 URI |
| 024 | 7 | a https://doi.org/10.1111/bcpt.128752 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1379647342 URI |
| 040 | a (SwePub)uud (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Sjöholm, Louise Ku Karolinska Institutet4 aut |
| 245 | 1 0 | a Evaluation of Post-Mortem Effects on Global Brain DNA Methylation and Hydroxymethylation. |
| 264 | c 2017-11-13 | |
| 264 | 1 | b Wiley,c 2018 |
| 338 | a print2 rdacarrier | |
| 520 | a The number of epigenetic studies on brain functions and diseases are dramatically increasing, but little is known about the impact of post-mortem intervals and post-sampling effects on DNA modifications such as 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Here, we examined post-mortem-induced changes in global brain 5mC and 5hmC levels at post-mortem intervals up to 540 min., and studied effects of tissue heat stabilization, using LUMA and ELISA. The global 5mC and 5hmC levels were generally higher in the cerebellum of adult rats than neonates. When measured by ELISA, the global 5mC content in adults, but not neonates, decreased with the post-mortem interval reaching a significantly lower level in cerebellum tissue at the post-mortem interval 540 min. (2.9 ± 0.7%; mean ± S.E.M.) compared to control (3.7 ± 0.6%). The global 5hmC levels increased with post-mortem interval reaching a significantly higher level at 540 min. (0.29 ± 0.06%) compared to control (0.19 ± 0.03%). This suggests that the post-mortem interval may confound 5mC and 5hmC analysis in human brain tissues as the post-mortem handling could vary substantially. The reactive oxygen species (ROS) level in cerebellum also increased over time, in particular in adults, and may be part of the mechanism that causes the observed post-mortem changes in 5mC and 5hmC. The global 5mC and 5hmC states were unaffected by heat stabilization, allowing analysis of tissues that are stabilized to preserve more labile analytes. Further studies in human samples are needed to confirm post-mortem effects on DNA methylation/hydroxymethylation and elucidate details of the underlying mechanisms. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Neurovetenskaper0 (SwePub)301052 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Neurosciences0 (SwePub)301052 hsv//eng |
| 700 | 1 | a Ransome, Yusufu Harvard T.H. Chan School of Public Health, Boston, USA4 aut |
| 700 | 1 | a Ekström, Tomas Ju Karolinska Institutet4 aut |
| 700 | 1 | a Karlsson, Oskaru Uppsala universitet,Institutionen för farmaceutisk biovetenskap4 aut0 (Swepub:uu)oskar255 |
| 710 | 2 | a Karolinska Institutetb Harvard T.H. Chan School of Public Health, Boston, USA4 org |
| 773 | 0 | t Basic & Clinical Pharmacology & Toxicologyd : Wileyg 122:2, s. 208-213q 122:2<208-213x 1742-7835x 1742-7843 |
| 856 | 4 | u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/bcpt.12875 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-342430 |
| 856 | 4 8 | u https://doi.org/10.1111/bcpt.12875 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:137964734 |
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