Common Genetic Variations in the NALP3 Inflammasome Are Associated with Delayed Apoptosis of Human Neutrophils

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Common Genetic Variations in the NALP3 Inflammasome Are Associated with Delayed Apoptosis of Human Neutrophils

Blomgran, Robert (author): Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet,Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden

Brodin Patcha, Veronika (author): Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet,Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden

Verma, Deepti (author): Linköpings universitet,Cellbiologi,Hälsouniversitetet,Division of Cell Biology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden

Bergström, Ida (author): Linköpings universitet,Kardiologi,Hälsouniversitetet,Division of Cardiovascular Medicine, Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University, Linköping, Sweden

Söderkvist, Peter (author): Linköpings universitet,Cellbiologi,Hälsouniversitetet,Division of Cell Biology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden

Sjöwall, Christoffer (author): Östergötlands Läns Landsting,Linköpings universitet,Reumatologi,Hälsouniversitetet,Reumatologiska kliniken i Östergötland,Division of Rheumatology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden

Eriksson, Per (author): Östergötlands Läns Landsting,Linköpings universitet,Reumatologi,Hälsouniversitetet,Njurmedicinska kliniken US,Division of Rheumatology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden

Lerm, Maria (author): Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet,Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Center for Infectious Medicine, Karolinska Institute Huddinge, Stockholm, Sweden

Stendahl, Olle (author): Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet,Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden

Särndahl, Eva, 1963- (author): Örebro universitet,Institutionen för hälsovetenskap och medicin,Department of Clinical Medicine, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden

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2012-03-05
2012
English.
In: PLOS ONE. - San Francisco, USA : Public Library of Science. - 1932-6203. ; 7:3

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Journal article (peer-reviewed)

Abstract Subject headings

Background: Neutrophils are key-players in the innate host defense and their programmed cell death and removal are essential for efficient resolution of inflammation. These cells recognize a variety of pathogens, and the NOD-like receptors (NLRs) have been suggested as intracellular sensors of microbial components and cell injury/stress. Some NLR will upon activation form multi-protein complexes termed inflammasomes that result in IL-1 beta production. NLR mutations are associated with auto-inflammatory syndromes, and our previous data propose NLRP3 (Q705K)/CARD-8 (C10X) polymorphisms to contribute to increased risk and severity of inflammatory disease by acting as genetic susceptibility factors. These gene products are components of the NALP3 inflammasome, and approximately 6.5% of the Swedish population are heterozygote carriers of these combined gene variants. Since patients carrying the Q705K/C10X polymorphisms display leukocytosis, the aim of the present study was to find out whether the inflammatory phenotype was related to dysfunctional apoptosis and impaired clearance of neutrophils by macrophages. less thanbrgreater than less thanbrgreater thanMethods and Findings: Patients carrying the Q705K/C10X polymorphisms displayed significantly delayed spontaneous as well as microbe-induced apoptosis compared to matched controls. Western blotting revealed increased levels and phosphorylation of Akt and Mcl-1 in the patients neutrophils. In contrast to macrophages from healthy controls, macrophages from the patients produced lower amounts of TNF; suggesting impaired macrophage clearance response. less thanbrgreater than less thanbrgreater thanConclusions: The Q705K/C10X polymorphisms are associated with delayed apoptosis of neutrophils. These findings are explained by altered involvement of different regulators of apoptosis, resulting in an anti-apoptotic profile. Moreover, the macrophage response to ingestion of microbe-induced apoptotic neutrophils is altered in the patients. Taken together, the patients display impaired turnover and clearance of apoptotic neutrophils, pointing towards a dysregulated innate immune response that influences the resolution of inflammation. The future challenge is to understand how microbes affect the activation of inflammasomes, and why this interaction will develop into severe inflammatory disease in certain individuals.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)

Keyword

MEDICINE
MEDICIN
Medicine

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By the author/editor: Blomgran, Robert; Brodin Patcha, V ...; Verma, Deepti; Bergström, Ida; Söderkvist, Pete ...; Sjöwall, Christo ...; show more...; Eriksson, Per; Lerm, Maria; Stendahl, Olle; Särndahl, Eva, 1 ...; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Rheumatology and ...

Articles in the publication: PLOS ONE

By the university: Linköping University; Örebro University; Karolinska Institutet

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