WFRF:(Strichman Almashanu LZ)
Search: WFRF:(Strichman Almashanu LZ) > A genome-wide scree...
- 1 of 1
- Previous record
- Next record
- To hitlist
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03571naa a2200325 4500 | |
| 001 | oai:prod.swepub.kib.ki.se:18244613 | |
| 003 | SwePub | |
| 008 | 240811s2002 | |||||||||||000 ||eng| | |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:182446132 URI |
| 024 | 7 | a https://doi.org/10.1101/gr.2241022 DOI |
| 040 | a (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Strichman-Almashanu, LZ4 aut |
| 245 | 1 0 | a A genome-wide screen for normally methylated human CpG islands that can identify novel imprinted genes |
| 264 | c 2002-03-20 | |
| 264 | 1 | b Cold Spring Harbor Laboratory,c 2002 |
| 520 | a DNA methylation is a covalent modification of the nucleotide cytosine that is stably inherited at the dinucleotide CpG by somatic cells, and 70% of CpG dinucleotides in the genome are methylated. The exception to this pattern of methylation are CpG islands, CpG-rich sequences that are protected from methylation, and generally are thought to be methylated only on the inactive X-chromosome and in tumors, as well as differentially methylated regions (DMRs) in the vicinity of imprinted genes. To identify chromosomal regions that might harbor imprinted genes, we devised a strategy for isolating a library of normally methylated CpG islands. Most of the methylated CpG islands represented high copy number dispersed repeats. However, 62 unique clones in the library were characterized, all of which were methylated and GC-rich, with a GC content >50%. Of these, 43 clones also showed a CpGobs/CpGexp >0.6, of which 30 were studied in detail. These unique methylated CpG islands mapped to 23 chromosomal regions, and 12 were differentially methylated regions in uniparental tissues of germline origin, i.e., hydatidiform moles (paternal origin) and complete ovarian teratomas (maternal origin), even though many apparently were methylated in somatic tissues. We term these sequences gDMRs, for germline differentially methylated regions. At least two gDMRs mapped near imprinted genes, HYMA1 and a novel homolog of Elongin A and Elongin A2, which we termElongin A3. Surprisingly, 18 of the methylated CpG islands were methylated in germline tissues of both parental origins, representing a previously uncharacterized class of normally methylated CpG islands in the genome, and which we term similarly methylated regions (SMRs). These SMRs, in contrast to the gDMRs, were significantly associated with telomeric band locations (P = .0008), suggesting a potential role for SMRs in chromosome organization. At least 10 of the methylated CpG islands were on average 85% conserved between mouse and human. These sequences will provide a valuable resource in the search for novel imprinted genes, for defining the molecular substrates of the normal methylome, and for identifying novel targets for mammalian chromatin formation.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos.AF484557–AF484583.] | |
| 700 | 1 | a Lee, RS4 aut |
| 700 | 1 | a Onyango, PO4 aut |
| 700 | 1 | a Perlman, E4 aut |
| 700 | 1 | a Flam, F4 aut |
| 700 | 1 | a Frieman, MB4 aut |
| 700 | 1 | a Feinberg, AP4 aut |
| 773 | 0 | t Genome researchd : Cold Spring Harbor Laboratoryg 12:4, s. 543-554q 12:4<543-554x 1088-9051x 1549-5469 |
| 856 | 4 | u http://genome.cshlp.org/content/12/4/543.full.pdf |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:18244613 |
| 856 | 4 8 | u https://doi.org/10.1101/gr.224102 |
Find in a library
- Genome research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Strichman-Almash ...
- Lee, RS
- Onyango, PO
- Perlman, E
- Flam, F
- Frieman, MB
- show more...
- Feinberg, AP
- show less...
- Articles in the publication
- Genome research
- By the university
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
