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Effects of freeze-t...
Effects of freeze-thawing and intravenous infusion on mesenchymal stromal cell gene expression.
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- Hoogduijn, Martin J (författare)
- Erasmus University Medical Center
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- de Witte, Samantha (författare)
- Erasmus University Medical Center
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- Luk, Franka (författare)
- Erasmus University Medical Center
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- van den Hout-van Vroonhoven, Mirjam Cgn (författare)
- Erasmus University Medical Center
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- Ignatowicz, Lech (författare)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Catar, Rusan (författare)
- Charité - University Medicine Berlin
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- Strini, Tanja (författare)
- Erasmus University Medical Center
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- Korevaar, Sander S (författare)
- Erasmus University Medical Center
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van IJcken, Wilfred Fj (författare)
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- Betjes, Michiel Gh (författare)
- Erasmus University Medical Center
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- Franquesa, Marcella (författare)
- Erasmus University Medical Center
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- Moll, Guido (författare)
- Karolinska Institutet
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- Baan, Carla C (författare)
- Erasmus University Medical Center
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2016
- 2016
- Engelska.
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Ingår i: Stem Cells and Development. - : Mary Ann Liebert Inc. - 1557-8534 .- 1547-3287.
- Relaterad länk:
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http://www.ncbi.nlm....
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Mesenchymal stromal cells (MSC) are increasingly used as an investigative therapeutic product for immune disorders and degenerative disease. Typically, MSC are isolated from human tissue, expanded in culture and cryopreserved until usage. The safety and efficacy of MSC therapy will depend on the phenotypical and functional characteristics of MSC. The freeze-thawing procedure may change these characteristics. Furthermore, the cells encounter a microenvironment after administration that may impact their properties. It has been demonstrated that the majority of MSC localize to the lungs after intravenous infusion, making this the site to study the effects of the in vivo milieu on administered MSC. In the present study we investigated the effect of freeze-thawing and the mouse lung microenvironment on human adipose tissue-derived MSC. There were effects of freeze-thawing on the whole genome expression profile of MSC, although the effects did not exceed inter-donor differences. There were no major changes in the expression of hemostatic regulators on transcriptional level, but significantly increased expression of procoagulant tissue factor on the surface of thawed adipose MSC, correlating with increased procoagulant activity of thawed cells. Exposure for 2h to the lung microenvironment had a major effect on MSC gene expression and affected several immunological pathways. This indicates that MSC undergo functional changes shortly after infusion and this may influence the efficacy of MSC to modulate inflammatory responses. The results of this study demonstrate that MSC rapidly alter in response to the local milieu and that disease specific conditions may shape MSC after administration.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
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Hoogduijn, Marti ...
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de Witte, Samant ...
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Luk, Franka
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van den Hout-van ...
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Ignatowicz, Lech
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Catar, Rusan
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visa fler...
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Strini, Tanja
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Korevaar, Sander ...
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van IJcken, Wilf ...
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Betjes, Michiel ...
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Franquesa, Marce ...
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Moll, Guido
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Baan, Carla C
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