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Sökning: WFRF:(Sundler M) > (2010-2014) > A Major Lineage of ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004597naa a2200541 4500
001oai:lup.lub.lu.se:c0c7dc92-8749-4053-9fd8-63087aa33be2
003SwePub
008160404s2012 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/31607392 URI
0247 a https://doi.org/10.1210/en.2012-15952 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Egerod, Kristoffer L4 aut
2451 0a A Major Lineage of Enteroendocrine Cells Coexpress CCK, Secretin, GIP, GLP-1, PYY, and Neurotensin but Not Somatostatin.
264 c 2012-12-01
264 1b The Endocrine Society,c 2012
338 a electronic2 rdacarrier
520 a Enteroendocrine cells such as duodenal cholecystokinin (CCK cells) are generally thought to be confined to certain segments of the gastrointestinal (GI) tract and to store and release peptides derived from only a single peptide precursor. In the current study, however, transgenic mice expressing enhanced green fluorescent protein (eGFP) under the control of the CCK promoter demonstrated a distribution pattern of CCK-eGFP positive cells that extended throughout the intestine. Quantitative PCR and liquid chromatography-mass spectrometry proteomic analyses of isolated, FACS-purified CCK-eGFP-positive cells demonstrated expression of not only CCK but also glucagon-like peptide 1 (GLP-1), gastric inhibitory peptide (GIP), peptide YY (PYY), neurotensin, and secretin, but not somatostatin. Immunohistochemistry confirmed this expression pattern. The broad coexpression phenomenon was observed both in crypts and villi as demonstrated by immunohistochemistry and FACS analysis of separated cell populations. Single-cell quantitative PCR indicated that approximately half of the duodenal CCK-eGFP cells express one peptide precursor in addition to CCK, whereas an additional smaller fraction expresses two peptide precursors in addition to CCK. The coexpression pattern was further confirmed through a cell ablation study based on expression of the human diphtheria toxin receptor under the control of the proglucagon promoter, in which activation of the receptor resulted in a marked reduction not only in GLP-1 cells, but also PYY, neurotensin, GIP, CCK, and secretin cells, whereas somatostatin cells were spared. Key elements of the coexpression pattern were confirmed by immunohistochemical double staining in human small intestine. It is concluded that a lineage of mature enteroendocrine cells have the ability to coexpress members of a group of functionally related peptides: CCK, secretin, GIP, GLP-1, PYY, and neurotensin, suggesting a potential therapeutic target for the treatment and prevention of diabetes and obesity.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng
7001 a Engelstoft, Maja S4 aut
7001 a Grunddal, Kaare V4 aut
7001 a Nøhr, Mark K4 aut
7001 a Secher, Anna4 aut
7001 a Sakata, Ichiro4 aut
7001 a Pedersen, Jens4 aut
7001 a Windeløv, Johanne A4 aut
7001 a Füchtbauer, Ernst-Martin4 aut
7001 a Olsen, Jørgen4 aut
7001 a Sundler, Franku Lund University,Lunds universitet,Neuroendokrin cellbiologi,Forskargrupper vid Lunds universitet,Neuroendocrine Cell Biology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-fsu
7001 a Christensen, Jan P4 aut
7001 a Wierup, Nilsu Lund University,Lunds universitet,Neuroendokrin cellbiologi,Forskargrupper vid Lunds universitet,Neuroendocrine Cell Biology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-nwi
7001 a Olsen, Jesper V4 aut
7001 a Holst, Jens J4 aut
7001 a Zigman, Jeffrey M4 aut
7001 a Poulsen, Steen S4 aut
7001 a Schwartz, Thue W4 aut
7102 a Neuroendokrin cellbiologib Forskargrupper vid Lunds universitet4 org
7730 t Endocrinologyd : The Endocrine Societyx 0013-7227x 1945-7170
8564 u https://portal.research.lu.se/files/5195065/3327020.pdfx primaryx freey FULLTEXT
8564 u http://www.ncbi.nlm.nih.gov/pubmed/23064014?dopt=Abstracty FULLTEXT
8564 u http://dx.doi.org/10.1210/en.2012-1595y FULLTEXT
8564 u https://lup.lub.lu.se/search/files/5195065/3327020.pdf
8564 8u https://lup.lub.lu.se/record/3160739
8564 8u https://doi.org/10.1210/en.2012-1595

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