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Dissection of transient oxidative stress response in Saccharomyces cerevisiae by using DNA microarrays

Koerkamp, M. G. (author)
Rep, M. (author)
Bussemaker, H. J. (author)
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Hardy, Gpma (author)
Mul, A. (author)
Piekarska, K. (author)
Al-Khalili Szigyarto, Cristina (author)
KTH,Bioteknologi
de Mattos, J. M. T. (author)
Tabak, H. F. (author)
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 (creator_code:org_t)
2002
2002
English.
In: Molecular Biology of the Cell. - 1059-1524 .- 1939-4586. ; 13:8, s. 2783-2794
Related links:
https://urn.kb.se/re...
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https://doi.org/10.1...
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Yeast cells were grown in glucose-limited chemostat cultures and forced to switch to a new carbon source, the fatty acid oleate. Alterations in gene expression were monitored using DNA microarrays combined with bioinformatics tools, among which was included the recently developed algorithm REDUCE. Immediately after the switch to oleate, a transient and very specific stress response was observed, followed by the up-regulation of genes encoding peroxisomal enzymes required for fatty acid metabolism. The stress response included up-regulation of genes coding for enzymes to keep thioredoxin and glutathione reduced, as well as enzymes required for the detoxification of reactive oxygen species. Among the genes coding for various isoenzymes involved in these processes, only a specific subset was expressed. Not the general stress transcription factors Msn2 and Msn4, but rather the specific factor Yap1p seemed to be the main regulator of the stress response. We ascribe the initiation of the oxidative stress response to a combination of poor redox flux and fatty acid-induced uncoupling of the respiratory chain during the metabolic reprogramming phase.

Keyword

old-yellow enzyme
gene-expression
transcription factors
peroxisome proliferation
hydrogen-peroxide
binding protein
fatty-acids
yeast
induction
genome

Publication and Content Type

ref (subject category)
art (subject category)

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