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Reusable hydroxyapa...
Reusable hydroxyapatite nanocrystal sensors for protein adsorption
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- Tagaya, M. (författare)
- Tokyo Institute of Technology,National Institute for Materials Science (NIMS)
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- Ikoma, T. (författare)
- Tokyo Institute of Technology,National Institute for Materials Science (NIMS)
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- Hanagata, N. (författare)
- National Institute for Materials Science (NIMS)
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- Chakarov, Dinko, 1955 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kasemo, Bengt Herbert, 1942 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Tanaka, J. (författare)
- Tokyo Institute of Technology
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(creator_code:org_t)
- 2016-01-12
- 2010
- Engelska.
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Ingår i: Science and Technology of Advanced Materials. - : Informa UK Limited. - 1468-6996 .- 1878-5514. ; 11:4
- Relaterad länk:
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http://dx.doi.org/10...
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https://www.tandfonl...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- The repeatability of the adsorption and removal of fibrinogen and fetal bovine serum on hydroxyapatite (HAp) nanocrystal sensors was investigated by Fourier transform infrared (FTIR) spectroscopy and quartz crystal microbalance with dissipation (QCM-D) monitoring technique. The HAp nanocrystals were coated on a gold-coated quartz sensor by electrophoretic deposition. Proteins adsorbed on the HAp sensors were removed by (i) ammonia/hydrogen peroxide mixture (APM), (ii) ultraviolet light (UV), (iii) UV/APM, (iv) APM/UV and (v) sodium dodecyl sulfate (SDS) treatments. FTIR spectra of the reused surfaces revealed that the APM and SDS treatments left peptide fragments or the proteins adsorbed on the surfaces, whereas the other methods successfully removed the proteins. The QCM-D measurements indicated that in the removal treatments, fibrinogen was slowly adsorbed in the first cycle because of the change in surface wettability revealed by contact angle measurements. The SDS treatment was not effective in removing proteins. The APM or UV treatment decreased the frequency shifts for the reused HAp sensors. The UV/APM treatment did not induce the frequency shifts but decreased the dissipation shifts. Therefore, we conclude that the APM/UV treatment is the most useful method for reproducing protein adsorption behavior on HAp sensors.
Ämnesord
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Nyckelord
- quartz crystal microbalance
- protein adsorption
- thin layer
- hydroxyapatite sensor
- reusability
Publikations- och innehållstyp
- kon (ämneskategori)
- ref (ämneskategori)
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