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Sökning: WFRF:(Urrea S.) > (2015) > Uptake and eliminat...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003745naa a2200553 4500
001oai:gup.ub.gu.se/224099
003SwePub
008240528s2015 | |||||||||||000 ||eng|
024a https://gup.ub.gu.se/publication/2240992 URI
024a https://doi.org/10.3109/17435390.2014.9637242 DOI
040 a (SwePub)gu
041 a eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Ribeiro, F.4 aut
2451 0a Uptake and elimination kinetics of silver nanoparticles and silver nitrate by Raphidocelis subcapitata: The influence of silver behaviour in solution
264 c 2014-10-13
264 1b Informa UK Limited,c 2015
520 a Raphidocelis subcapitata is a freshwater algae species that constitutes the basis of many aquatic trophic chains. In this study, R. subcapitata was used as a model species to investigate the kinetics of uptake and elimination of silver nanoparticles (AgNP) in comparison to silver nitrate (AgNO3) with particular focus on the Ag sized-fractions in solution. AgNP used in this study were provided in a suspension of 1mg Ag/l, with an initial size of 3-8nm and coated with an alkane material. Algae was exposed for 48 h to both AgNP and AgNO3 and sampled at different time points to determine their internal Ag concentration over time. Samples were collected and separated into different sized fractions: total (Ag-tot), water column Ag (Ag-water), small particulate Ag (Ag-small.part.) and dissolved Ag (Ag-dis). At AgNO3 exposures algae reached higher bioconcentration factor (BCF) and lower elimination rate constants than at AgNP exposures, meaning that Ag is more readily taken up by algae in its dissolved form than in its small particulate form, however slowly eliminated. When modelling the kinetics based on the Ag-dis fraction, a higher BCF was found. This supports our hypothesis that Ag would be internalised by algae only in its dissolved form. In addition, algae images obtained by Coherent Anti-stokes Raman Scattering (CARS) microscopy demonstrated large aggregates of nanoparticles external to the algae cells with no evidence of its internalisation, thus providing a strong suggestion that these AgNP were not able to penetrate the cells and Ag accumulation happens through the uptake of Ag ions.
650 7a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng
653 a Bioconcentration factor
653 a Raphidocelis subcapitata
653 a silver nanoparticles
653 a toxicokinetics
653 a 2 green-algae
653 a extracellular polymeric substances
653 a raman scattering
653 a microscopy
653 a pseudokirchneriella-subcapitata
653 a daphnia-magna
653 a chlamydomonas-reinhardtii
653 a toxicity
653 a stability
653 a bioassays
653 a chloride
653 a Science & Technology - Other Topics
653 a Toxicology
700a Gallego-Urrea, Julian A.,d 1977u Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology4 aut0 (Swepub:gu)xurrju
700a Goodhead, R. M.4 aut
700a Van Gestel, C. A. M.4 aut
700a Moger, J.4 aut
700a Soares, Amvm4 aut
700a Loureiro, S.4 aut
710a Göteborgs universitetb Institutionen för kemi och molekylärbiologi4 org
773t Nanotoxicologyd : Informa UK Limitedg 9:6, s. 686-695q 9:6<686-695x 1743-5390x 1743-5404
8564 8u https://gup.ub.gu.se/publication/224099
8564 8u https://doi.org/10.3109/17435390.2014.963724

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