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Somatic hybridizati...
Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis
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- Waara, Sylvia, 1958- (author)
- Department of Plant Physiology, Uppsala University, Uppsala, Sweden
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- Tegelström, H. (author)
- Department of Plant Physiology, Uppsala University, Uppsala, Sweden
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- Wallin, Anita (author)
- Department of Plant Physiology, Uppsala University, Uppsala, Sweden
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- Eriksson, Tage (author)
- Department of Plant Physiology, Uppsala University, Uppsala, Sweden
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(creator_code:org_t)
- Heidelberg : Springer Berlin/Heidelberg, 1989
- 1989
- English.
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In: Theoretical and Applied Genetics. - Heidelberg : Springer Berlin/Heidelberg. - 0040-5752 .- 1432-2242. ; 77:1, s. 49-56
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Green mesophyll protoplasts of the dihaploid potato line 198∶2 (Solanum tuberosum L.) were fused with herbicide-bleached mesophyll protoplasts of the dihaploid potato line 67∶9 using a polyethylene glycol protocol. Heterokaryons were identified under a fluorescence microscope using the dual fluorescence of carboxyfluorescein-stained, herbicide-bleached protoplasts and the autofluorescence of green mesophyll protoplasts. About 20% of the protoplasts survived the fusion treatment, and the fusion frequency was 3%-4%. Unfused and fused protoplasts were mass cultured for 6 weeks after which vigorously growing calli were selected and transferred to shoot regeneration medium. Somatic hybrids were identified by a combination of five isozyme markers, and the ploidy level was determined by flow cytometry. Out of 15 calli that regenerated shoots, 6 plants derived from 2 different calli were identified as hexaploid somatic hybrids, while one morphologically deviant plant from a third callus was identified as a mixoploid that had lost some enzyme markers after 4 months of culturing.
Subject headings
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
Keyword
- Isozymes
- Ploidy level
- Protoplast fusion
- Solanum tuberosum
- Somatic hybrids
Publication and Content Type
- ref (subject category)
- art (subject category)
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