WFRF:(Wallace W)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03377naa a2200397 4500 | |
| 001 | oai:lup.lub.lu.se:d967b92c-0b29-43bd-a682-71dc908898d9 | |
| 003 | SwePub | |
| 008 | 160401s1989 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/11049092 URI |
| 024 | 7 | a https://doi.org/10.1038/nbt0989-9342 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Larrick, James W4 aut |
| 245 | 1 0 | a Polymemse chain reaction using mixed primers. Cloning of human monoclonal antibody variable region genes from single hybridoma cells |
| 264 | c 1989-09-01 | |
| 264 | 1 | b Springer Science and Business Media LLC,c 1989 |
| 520 | a We describe a general approach to rapidly obtain the DNA sequence encoding the variable region of any immunoglobulin chain using the polymerase chain reaction and a mixture of upstream primers corresponding to the leader sequence, and one downstream primer designed from the conserved nucleotide sequence of the constant region. The approach was applied to five different hybridomas producing human monoclonal antibodies and variable regions for both bold gamma and mu heavy chain and kappa and lambda light chain genes were successfully cloned. cDNA encoding variable regions could be amplified from single hybridoma cells isolated by micromanipulation. This approach will permit analysis of B cell clonal ontogeny, antibody diversity and lymphoma cell progression and heterogeneity. It will also facilitate structural and functional studies of immunoglobulins as well as the rapid construction of chimeric antibodies. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmakologi och toxikologi0 (SwePub)301022 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmacology and Toxicology0 (SwePub)301022 hsv//eng |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Läkemedelskemi0 (SwePub)301032 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medicinal Chemistry0 (SwePub)301032 hsv//eng |
| 700 | 1 | a Danielsson, Lena4 aut |
| 700 | 1 | a Brenner, Carol A4 aut |
| 700 | 1 | a Wallace, Ellen F4 aut |
| 700 | 1 | a Abrahamson, Magnusu Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)kkem-mab |
| 700 | 1 | a Fry, Kirk E4 aut |
| 700 | 1 | a Borrebaeck, Carlu Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)immt-cbo |
| 710 | 2 | a Avdelningen för klinisk kemi och farmakologib Institutionen för laboratoriemedicin4 org |
| 773 | 0 | t Bio/Technologyd : Springer Science and Business Media LLCg 7:9, s. 934-938q 7:9<934-938x 0733-222X |
| 773 | 0 | t Nature Biotechnologyd : Springer Science and Business Media LLCg 7:9, s. 934-938q 7:9<934-938x 1087-0156x 1546-1696 |
| 856 | 4 | u http://dx.doi.org/10.1038/nbt0989-934y FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/1104909 |
| 856 | 4 8 | u https://doi.org/10.1038/nbt0989-934 |
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