WFRF:(Warner John C.)
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Optical and electrochemical detection of DNA
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- Downs, Mark E.A. (author)
- Cranfield Institute of Technology, UK
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- Warner, Philip J. (author)
- Cranfield Institute of Technology, UK
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- Turner, Anthony (author)
- Cranfield University, UK
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- (creator_code:org_t)
- Elsevier, 1988
- 1988
- English.
- In: Biomaterials. - : Elsevier. - 0142-9612 .- 1878-5905. ; 9:1, s. 66-70
- Journal article (peer-reviewed)
Abstract
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- There is a growing demand for the production of a DNA biosensor with applications in medicine, the food industry, agriculture, veterinary science and environmental science. In this paper we describe methods for the optical and electrochemical detection of DNA using the enzyme horseradish peroxidase (EC 1.11.1.7) and glucose oxidase (EC 1.1.3.4). We have used bis-methylacridinium nitrate and luminol for the optical detection of DNA using a purpose built, inexpensive luminometer. Using this system detection limits of 10−11g of plasmid DNA have been observed. Electrochemical detection of DNA was carried out by the use of a fluoride ion selective electrode and stripping voltametry. DNA was detected down to 1 (10−9 − 10−10g of DNA by the enzymatic release of halogen ions from organohalogen compounds.
Keyword
- DNA
- luminescence
- DNA biosensor
- electrochemical detection
- glucose oxidase. horseradish peroxidase. stripping
- voltametly. ion selective electrodes
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- ref (subject category)
- art (subject category)
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