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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00006667naa a2200853 4500
001oai:gup.ub.gu.se/237504
003SwePub
008240910s2016 | |||||||||||000 ||eng|
009oai:DiVA.org:his-11781
0247 a https://gup.ub.gu.se/publication/2375042 URI
0247 a https://doi.org/10.1007/s12015-015-9621-92 DOI
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-117812 URI
040 a (SwePub)gud (SwePub)his
041 a eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Asplund, Annika,d 1979-u Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden,Bioinformatik, Bioinformatics4 aut0 (Swepub:his)aspa
2451 0a One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells
264 c 2015-09-19
264 1b Springer Science and Business Media LLC,c 2016
520 a Human hepatocytes display substantial functional inter-individual variation regarding drug metabolizing functions. In order to investigate if this diversity is mirrored in hepatocytes derived from different human pluripotent stem cell (hPSC) lines, we evaluated 25 hPSC lines originating from 24 different donors for hepatic differentiation and functionality. Homogenous hepatocyte cultures could be derived from all hPSC lines using one standardized differentiation procedure. To the best of our knowledge this is the first report of a standardized hepatic differentiation procedure that is generally applicable across a large panel of hPSC lines without any adaptations to individual lines. Importantly, with regard to functional aspects, such as Cytochrome P450 activities, we observed that hepatocytes derived from different hPSC lines displayed inter-individual variation characteristic for primary hepatocytes obtained from different donors, while these activities were highly reproducible between repeated experiments using the same line. Taken together, these data demonstrate the emerging possibility to compile panels of hPSC-derived hepatocytes of particular phenotypes/genotypes relevant for drug metabolism and toxicity studies. Moreover, these findings are of significance for applications within the regenerative medicine field, since our stringent differentiation procedure allows the derivation of homogenous hepatocyte cultures from multiple donors which is a prerequisite for the realization of future personalized stem cell based therapies.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicin0 (SwePub)3022 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicine0 (SwePub)3022 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
653 a Hepatocyte differentiation
653 a Human induced pluripotent stem cells
653 a Human embyronic stem cells
653 a efficient differentiation
653 a epigenetic memory
653 a liver development
653 a hepatic
653 a endoderm
653 a lines
653 a expression
653 a induction
653 a enzyme
653 a donor
653 a hepatotoxicity
653 a Cell Biology
653 a Research & Experimental Medicine
653 a ates of america
653 a v111
653 a p16772
653 a ates of america
653 a v109
653 a p12538
653 a Cellular therapy
653 a Medical sciences
653 a Bioinformatik
7001 a Pradip, A.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden / Novo Nordisk A/S, Bagsværd, Denmark4 aut
7001 a Van Giezen, M.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Aspegren, A.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Choukair, H.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Rehnstrom, M.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Ghosheh, Nidalu Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatik, Bioinformatics4 aut0 (Swepub:his)ghon
7001 a El Hajjam, D.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Holmgren, Sandrau Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden / Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden4 aut0 (Swepub:gu)xhosan
7001 a Larsson, S.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Benecke, J.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Butron, M.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Wigander, A.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Noaksson, Karinu Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Sartipy, Peteru Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,AstraZeneca R&D, GMD CVMD GMed, Mölndal, Sweden,Bioinformatik, Bioinformatics4 aut0 (Swepub:his)sarp
7001 a Bjorquist, P.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden / NovaHep AB, Gothenburg, Sweden4 aut
7001 a Edsbagge, J.u Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7001 a Küppers-Munther, Barbarau Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Takara Bio Europe AB (former Cellartis AB), Arvid Wallgrens Backe 20, 413 46, Gothenburg, Sweden4 aut0 (Swepub:his)kupb
7001 a Jacobsson, Susannau Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden4 aut
7102 a Högskolan i Skövdeb Institutionen för biovetenskap4 org
7730 t Stem Cell Reviews and Reportsd : Springer Science and Business Media LLCg 12:1, s. 90-104q 12:1<90-104x 1550-8943x 1558-6804
8564 8u https://gup.ub.gu.se/publication/237504
8564 8u https://doi.org/10.1007/s12015-015-9621-9
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-11781

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