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Organization of Rib...
Organization of Ribosomes and Nucleoids in Escherichia coli Cells during Growth and in Quiescence
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- Chai, Qian (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Singh, Bhupender (författare)
- Umeå universitet,Uppsala universitet,Institutionen för cell- och molekylärbiologi,Institutionen för fysik
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- Peisker, Kristin (författare)
- Uppsala universitet,Struktur- och molekylärbiologi
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- Metzendorf, Nicole (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Ge, Xueliang (författare)
- Uppsala universitet,Struktur- och molekylärbiologi
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- Dasgupta, Santanu (författare)
- Uppsala universitet,Mikrobiologi
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- Sanyal, Suparna (författare)
- Uppsala universitet,Struktur- och molekylärbiologi
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(creator_code:org_t)
- 2014
- 2014
- Engelska.
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Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 289:16, s. 11342-11352
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: We studied ribosome and nucleoid distribution in Escherichia coli under growth and quiescence. Results: Spatially segregated ribosomes and nucleoids show drastically altered distribution in stationary phase or when treated with drugs affecting translation, transcription, nucleoid-topology, or cytoskeleton. Ribosome inheritance in daughter cells is frequently unequal. Conclusion: Cellular growth processes modulate ribosome and nucleoid distribution. Significance: This provides insight into subcellular organization of molecular machines. We have examined the distribution of ribosomes and nucleoids in live Escherichia coli cells under conditions of growth, division, and in quiescence. In exponentially growing cells translating ribosomes are interspersed among and around the nucleoid lobes, appearing as alternative bands under a fluorescence microscope. In contrast, inactive ribosomes either in stationary phase or after treatment with translation inhibitors such as chloramphenicol, tetracycline, and streptomycin gather predominantly at the cell poles and boundaries with concomitant compaction of the nucleoid. However, under all conditions, spatial segregation of the ribosomes and the nucleoids is well maintained. In dividing cells, ribosomes accumulate on both sides of the FtsZ ring at the mid cell. However, the distribution of the ribosomes among the new daughter cells is often unequal. Both the shape of the nucleoid and the pattern of ribosome distribution are also modified when the cells are exposed to rifampicin (transcription inhibitor), nalidixic acid (gyrase inhibitor), or A22 (MreB-cytoskeleton disruptor). Thus we conclude that the intracellular organization of the ribosomes and the nucleoids in bacteria are dynamic and critically dependent on cellular growth processes (replication, transcription, and translation) as well as on the integrity of the MreB cytoskeleton.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Antibiotics
- Cell Division
- Cytoskeleton
- DNA Replication
- Ribosomes
- Subcellular Organelles
- Transcription
- Translation
- FtsZ Ring
- Nucleoid
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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