Adjuvant properties of a biocompatible thermo-responsive polymer of N-isopropylacrylamide in autoimmunity and arthritis

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Adjuvant properties of a biocompatible thermo-responsive polymer of N-isopropylacrylamide in autoimmunity and arthritis

Shakya, Akhilesh Kumar (author): Indian Institute of Technology Kanpur, Kanpur, India

Kumar, Ashok (author): Indian Institute of Technology Kanpur, Kanpur, India

Nandakumar, Kutty Selva, 1965- (author): Karolinska Institutet,Karolinska Institute, Stockholm, Sweden

(creator_code:org_t)

2011-05-04
2011
English.
In: Journal of the Royal Society Interface. - London : The Royal Society Publishing. - 1742-5689 .- 1742-5662. ; 8:65, s. 1748-1759

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Journal article (peer-reviewed)

Abstract Subject headings

To evaluate the thermo-responsive poly(N-isopropylacrylamide) (PNiPAAm) polymer as an adjuvant, we synthesized PNiPAAm through free radical polymerization and characterized it both in vitro and in vivo. The polymer when mixed with collagen type II (CII) induced antigen-specific autoimmunity and arthritis. Mice immunized with PNiPAAm-CII developed significant levels of CII-specific IgG response comprising major IgG subclasses. Antigen-specific cellular recall response was also enhanced in these mice, while negligible level of IFN-gamma was detected in splenocyte cultures, in vitro. PNiPAAm-CII-immunized arthritic mouse paws showed massive infiltration of immune cells and extensive damage to cartilage and bone. As determined by immunostaining, most of the CII protein retained its native configuration after injecting it with PNiPAAm in naive mice. Physical adsorption of CII and the high-molecular-weight form of moderately hydrophobic PNiPAAm induced a significant anti-CII antibody response. Similar to CII, mice immunized with PNiPAAm and ovalbumin (PNiPAAm-Ova) induced significant anti-ovalbumin antibody response. Comparable levels of serum IFN-gamma, IL-1beta and IL-17 were observed in ovalbumin-immunized mice with complete Freund, incomplete Freund (CFA and IFA) or PNiPAAm adjuvants. However, serum IL-4 levels were significantly higher in PNiPAAm-Ova and CFA-Ova groups compared with the IFA-Ova group. Thus, we show for the first time, biocompatible and biodegradable thermo-responsive PNiPAAm can be used as an adjuvant in several immunological applications as well as in better understanding of the autoimmune responses against self-proteins. © 2011 The Royal Society

Subject headings

MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)

Keyword

Arthritis
Autoimmunity
Biocompatibility
Collagen
Thermo-responsive polymer

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ref (subject category)
art (subject category)

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By the author/editor: Shakya, Akhilesh ...; Kumar, Ashok; Nandakumar, Kutt ...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomaterials Sci ...

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By the university: Halmstad University; Karolinska Institutet

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