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A low cost surface plasmon resonance biosensor using a laser line generator

Chen, R. (författare)
Wang, M. (författare)
Wang, S. (författare)
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Liang, Hao (författare)
Högskolan i Gävle,Elektronik
Hu, X. (författare)
Sun, X. (författare)
Zhu, J. (författare)
Ma, L. (författare)
Jiang, M. (författare)
Hu, J. (författare)
Li, J. (författare)
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 (creator_code:org_t)
Elsevier BV, 2015
2015
Engelska.
Ingår i: Optics Communications. - : Elsevier BV. - 0030-4018 .- 1873-0310. ; 349, s. 83-88
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Due to the instrument designed by using a common surface plasmon resonance biosensor is extremely expensive, we established a portable and cost-effective surface plasmon resonance biosensing system. It is mainly composed of laser line generator, P-polarizer, customized prism, microfluidic cell, and line Charge Coupled Device (CCD) array. Microprocessor PIC24FJ128GA006 with embedded A/D converter, communication interface circuit and photoelectric signal amplifier circuit are used to obtain the weak signals from the biosensing system. Moreover, the line CCD module is checked and optimized on the number of pixels, pixels dimension, output amplifier and the timing diagram. The micro-flow cell is made of stainless steel with a high thermal conductivity, and the microprocessor based Proportional-Integral-Derivative (PID) temperature-controlled algorithm was designed to keep the constant temperature (25 °C) of the sample solutions. Correspondingly, the data algorithms designed especially to this biosensing system including amplitude-limiting filtering algorithm, data normalization and curve plotting were programmed efficiently. To validate the performance of the biosensor, ethanol solution samples at the concentrations of 5%, 7.5%, 10%, 12.5% and 15% in volumetric fractions were used, respectively. The fitting equation ΔRU=-752987.265+570237.348×RI with the R-Square of 0.97344 was established by delta response units (ΔRUs) to refractive indexes (RI). The maximum relative standard deviation (RSD) of 4.8% was obtained. 

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Elektroteknik och elektronik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
NATURVETENSKAP  -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
NATURAL SCIENCES  -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)

Nyckelord

Amplitude-limiting filtering algorithm
Biosensor
Laser line generator
Microprocessor
Surface plasmon resonance
Algorithms
Amplifiers (electronic)
Analog to digital conversion
Biosensors
Charge coupled devices
Cost effectiveness
Microprocessor chips
Pixels
Plasmons
Proportional control systems
Refractive index
Resonance
Signal filtering and prediction
Stainless steel
Thermal conductivity
Two term control systems
Communication interface
Filtering algorithm
High thermal conductivity
Laser line generators
Photoelectric signals
Proportional integral derivatives
Relative standard deviations
Surface plasmon resonance biosensor

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