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A-to-I editing of m...
A-to-I editing of microRNAs in the mammalian brain increases during development
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- Ekdahl, Ylva (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Shahrabi Farahani, Hossein (author)
- KTH,Beräkningsbiologi, CB,Science for Life Laboratory, SciLifeLab
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Behm, Mårten (author)
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- Lagergren, Jens (author)
- KTH,Beräkningsbiologi, CB,Science for Life Laboratory, SciLifeLab
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- Öhman, Marie (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Behm, Mikaela (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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(creator_code:org_t)
- 2012-05-29
- 2012
- English.
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In: Genome Research. - : Cold Spring Harbor Laboratory. - 1088-9051 .- 1549-5469. ; 22:8, s. 1477-1487
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https://doi.org/10.1...
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Abstract
Subject headings
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- Adenosine-to-inosine (A-to-I) RNA editing targets double-stranded RNA stem-loop structures in the mammalian brain. It has previously been shown that miRNAs are substrates for A-to-I editing. For the first time, we show that for several definitions of edited miRNA, the level of editing increases with development, thereby indicating a regulatory role for editing during brain maturation. We use high-throughput RNA sequencing to determine editing levels in mature miRNA, from the mouse transcriptome, and compare these with the levels of editing in pri-miRNA. We show that increased editing during development gradually changes the proportions of the two miR-376a isoforms, which previously have been shown to have different targets. Several other miRNAs that also are edited in the seed sequence show an increased level of editing through development. By comparing editing of pri-miRNA with editing and expression of the corresponding mature miRNA, we also show an editing-induced developmental regulation of miRNA expression. Taken together, our results imply that RNA editing influences the miRNA repertoire during brain maturation.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- adenosine
- complementary DNA
- double stranded RNA
- genomic DNA
- inosine
- microRNA
- Molecular Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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