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Differentiation of ...
Differentiation of normal and cancer cells induced by sulfhydryl reduction : biochemical and molecular mechanisms
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Parasassi, T. (författare)
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Brunelli, R. (författare)
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Bracci-Laudiero, L. (författare)
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Greco, G. (författare)
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Gustafsson, A. C. (författare)
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Krasnowska, E. K. (författare)
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- Lundeberg, Joakim (författare)
- KTH,Genteknologi
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Lundeberg, T. (författare)
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Pittaluga, E. (författare)
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Romano, M. C. (författare)
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Serafino, A. (författare)
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(creator_code:org_t)
- 2005-05-27
- 2005
- Engelska.
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Ingår i: Cell Death and Differentiation. - : Springer Science and Business Media LLC. - 1350-9047 .- 1476-5403. ; 12:10, s. 1285-1296
- Relaterad länk:
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We examined the morphological, biochemical and molecular outcome of a nonspecific sulfhydryl reduction in cells, obtained by supplementation of N-acetyl-L-cysteine (NAC) in a 0.1-10 mM concentration range. In human normal primary keratinocytes and in colon and ovary carcinoma cells we obtained evidences for: (i) a dose-dependent inhibition of proliferation without toxicity or apoptosis; (ii) a transition from a proliferative mesenchymal morphology to cell-specific differentiated structures; (iii) a noticeable increase in cell-cell and cell-substratum junctions; (iv) a relocation of the oncogenic beta-catenin at the cell-cell junctions; (v) inhibition of microtubules aggregation; (vi) upregulation of differentiation-related genes including p53, heat shock protein 27 gene, N-myc downstream-regulated gene 1, E-cadherin, and down-regulation of cyclooxygenase-2; (vii) inhibition of c-Src tyrosine kinase. In conclusion, a thiol reduction devoid of toxicity as that operated by NAC apparently leads to terminal differentiation of normal and cancer cells through a pleiade of converging mechanisms, many of which are targets of the recently developed differentiation therapy.
Nyckelord
- colon carcinoma
- cyclooxygenase-2
- junctions
- ovary carcinoma
- proliferation
- c-Src
- redox regulation
- src kinase
- colorectal-cancer
- n-acetylcysteine
- colon-cancer
- dna-damage
- stem-cells
- gene
- apoptosis
- cytoskeleton
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Parasassi, T.
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Brunelli, R.
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Bracci-Laudiero, ...
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Greco, G.
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Gustafsson, A. C ...
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Krasnowska, E. K ...
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visa fler...
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Lundeberg, Joaki ...
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Lundeberg, T.
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Pittaluga, E.
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Romano, M. C.
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Serafino, A.
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visa färre...
- Artiklar i publikationen
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Cell Death and D ...
- Av lärosätet
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Kungliga Tekniska Högskolan